真核生物DNA复制和修复蛋白保真机制分析

Analysis of fidelity mechanisms with eukaryotic DNA replication and repair proteins.

作者信息

Kunkel T A, Bebenek K, Roberts J D, Fitzgerald M P, Thomas D C

机构信息

Laboratory of Molecular Genetics, National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709.

出版信息

Genome. 1989;31(1):100-3. doi: 10.1139/g89-019.

DOI:10.1139/g89-019

PMID:2687085

Abstract

We are investigating the mechanisms for producing or avoiding errors during DNA synthesis catalyzed by DNA replication and repair proteins purified from eukaryotic sources. Using assays that monitor the fidelity of a single round of DNA synthesis in vitro, we have defined the error frequency and mutational specificity of the four classes of animal cell DNA polymerases (alpha, beta, delta, gamma), and the fidelity of an SV40 origin-dependent DNA replication complex in extracts of HeLa cells.

摘要

我们正在研究从真核生物中纯化得到的DNA复制和修复蛋白催化DNA合成过程中产生或避免错误的机制。通过监测体外单轮DNA合成保真度的实验，我们已经确定了四类动物细胞DNA聚合酶（α、β、δ、γ）的错误频率和突变特异性，以及HeLa细胞提取物中SV40依赖原点的DNA复制复合物的保真度。