Department of Science Application and Dissemination, National Taichung University of Education, Taichung 403, Taiwan.
J Hazard Mater. 2011 Nov 30;196:166-72. doi: 10.1016/j.jhazmat.2011.09.015. Epub 2011 Sep 10.
Acinetobacter calcoaceticus YC210 has been isolated and its ability to remove Victoria Blue R (VBR) from aqueous solution was assessed. The effects of various factors on decolorization efficiency were investigated in a batch system. The decolorization efficiency was found to be optimal within a pH of 5-7 and increased with VBR concentration up to 450 mg/l with high efficiency (94.5%) in a short time. The decolorization efficiency was significantly affected by cell concentrations. The decolorization of VBR by A. calcoaceticus YC210 followed first order kinetics. The apparent kinetic parameters of the Lineweaver-Burk equation, R(VBR,max) and K(m), were calculated as 6.93 mg-VBR/g-cell/h and 175.8 mg/l, respectively. Based on the biodegradation products, VBR degradation by A. calcoaceticus YC210 involves a stepwise demethylation process to yield partially dealkylated VBR species. To our knowledge, this is the first report using microbes to remove VBR. It clearly demonstrates the dealkylation pathway of VBR degradation.
已经分离出嗜水气单胞菌 YC210 并评估了其从水溶液中去除维多利亚蓝 R（VBR）的能力。在分批系统中研究了各种因素对脱色效率的影响。发现脱色效率在 pH 值为 5-7 时最佳，并且随着 VBR 浓度的增加至 450 mg/L 时，具有高效率（94.5%），在短时间内即可实现。细胞浓度对脱色效率有显著影响。嗜水气单胞菌 YC210 对 VBR 的脱色遵循一级动力学。Lineweaver-Burk 方程的表观动力学参数，R(VBR,max)和 K(m)，分别计算为 6.93 mg-VBR/g-细胞/h 和 175.8 mg/L。根据生物降解产物，VBR 被嗜水气单胞菌 YC210 降解涉及逐步脱甲基过程，生成部分去烷基化的 VBR 物质。据我们所知，这是首次使用微生物去除 VBR 的报道。它清楚地表明了 VBR 降解的脱烷基途径。
