Department of Chemistry and Ilse Katz Institute for Nanotechnology, Ben Gurion University, Beer Sheva 84105, Israel.
J Colloid Interface Sci. 2011 Dec 15;364(2):428-34. doi: 10.1016/j.jcis.2011.08.078. Epub 2011 Sep 3.
Mesostructured silica films have attracted interest as potential platforms for sensing, molecular sieving, catalysis, and others. The fabrication of free-standing silica films on water, however, is challenging due to the need for scaffolding agents that would constitute effective templates. We describe the assembly of thin film at the air/water interface comprising quaternary silicates and polydiacetylene (PDA), a unique chromatic polymer forming two-dimensional conjugated networks, and the use of these films for biological sensing. PDA exhibits a dual role in the system-both as the amphiphilic matrix facilitating immobilization of the silicate colloidal units at the air/water interface and additionally a chromatic reporter that undergoes visible blue-red transitions, accompanied by fluorescence transformations, in the presence of analytes. We demonstrate the application of the silicate/PDA thin films for the detection of bacterial proliferation.
介孔硅薄膜作为传感、分子筛、催化等方面的潜在平台引起了人们的兴趣。然而，由于需要支架剂来构成有效的模板，因此在水面上制造独立的硅薄膜具有挑战性。我们描述了在空气/水界面处组装由季硅酸盐和聚二乙炔（PDA）组成的薄膜，PDA 是一种形成二维共轭网络的独特变色聚合物，以及这些薄膜在生物传感中的应用。PDA 在该体系中具有双重作用——既作为两亲性基质促进硅酸钠胶体单元在空气/水界面的固定，又作为显色报告基团，在存在分析物时，其颜色发生可见的蓝-红转变，同时伴随着荧光转变。我们展示了硅酸钠/PDA 薄膜在检测细菌增殖方面的应用。
