Key Laboratory of Molecular Biophysics of the Ministry of Education, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, PR China.
Bioresour Technol. 2011 Nov;102(22):10414-8. doi: 10.1016/j.biortech.2011.08.056. Epub 2011 Aug 22.
Biodiesel synthesis and conformation of Burkholderia cepacia lipase (BCL) were studied in 19 different room temperature ionic liquids (RTLLs) with a range of cation and anion structures. Overall, anion selection had a greater influence on biodiesel conversion than cation choice. RTILs containing Tf2N- and PF6- anions were suitable reaction media, while RTIL of [OmPy][BF4] was the best reaction medium with a biodiesel yield of 82.2±1.2%. RTILs with strong water miscible properties showed very low biodiesel yields. Conformational analysis by FT-IR revealed that higher biodiesel conversion in RTILs was correlated with a low tendency in α-helix content of BCL. An ultrasound-assisted biocatalysis process in RTILs was used to improve mass transfer rate, leading to 83% reduction of the reaction time for biodiesel production.
研究了 19 种不同室温离子液体（RTIL）中生物柴油的合成和伯克霍尔德氏菌脂肪酶（BCL）的构象，这些 RTIL 的阳离子和阴离子结构各异。总的来说，阴离子的选择比对生物柴油转化率的影响更大。含有 Tf2N-和 PF6-阴离子的 RTIL 是合适的反应介质，而 [OmPy][BF4] 的 RTIL 是最佳反应介质，生物柴油产率为 82.2±1.2%。具有强水溶性的 RTIL 表现出非常低的生物柴油产率。傅里叶变换红外光谱（FT-IR）的构象分析表明，RTIL 中较高的生物柴油转化率与 BCL 中α-螺旋含量的低趋势相关。在 RTIL 中使用超声波辅助的生物催化过程来提高传质速率，使生物柴油生产的反应时间减少了 83%。
