Department of Laboratory Medicine, Medical Academy, Lithuanian University of Health Sciences, Eivenių 2, Kaunas, Lithuania.
Medicina (Kaunas). 2011;47(5):278-83.
The aim was to estimate changes in the resistance rates of Pseudomonas aeruginosa (P. aeruginosa) strains isolated from patients treated in intensive care units of the largest university hospital.
Isolates were identified with the Phoenix ID system (Becton Dickinson, USA). The minimum inhibitory concentration (MIC) of ceftazidime, ciprofloxacin, and amikacin were determined by the E-test and evaluated following the recommendations of the Clinical Laboratory Standards Institute.
In 2003, the proportion of P. aeruginosa strains resistant to piperacillin was greatest followed by strains resistant gentamicin and ciprofloxacin. In 2008, the resistance rates markedly changed being the highest to ciprofloxacin. An increase in the resistance rates to ciprofloxacin (+24%, P<0.001) and ceftazidime (+8.3%, P<0.05) was documented. In 2003, there were 66.7% of P. aeruginosa strains sensitive to all antibiotics tested, and this percentage decreased to 47.5% in 2008 (P<0.05). During the study, a significant increase in the median MICs for ciprofloxacin and amikacin was observed (P<0.001); however, no significant change was documented for ceftazidime.
P. aeruginosa remains an important nosocomial pathogen with relatively high overall resistance to antimicrobial agents, and the resistance level is increasing.
评估从最大大学医院的重症监护病房治疗的患者中分离的铜绿假单胞菌（P. aeruginosa）菌株的耐药率变化。
使用 Phoenix ID 系统（美国贝克顿·迪金森公司）对分离株进行鉴定。采用 E 试验测定头孢他啶、环丙沙星和阿米卡星的最小抑菌浓度（MIC），并根据临床实验室标准协会的建议进行评估。
2003 年，对哌拉西林耐药的铜绿假单胞菌菌株比例最高，其次是对庆大霉素和环丙沙星耐药的菌株。2008 年，耐药率明显变化，对环丙沙星的耐药率最高。记录到对环丙沙星（+24%，P<0.001）和头孢他啶（+8.3%，P<0.05）的耐药率增加。2003 年，有 66.7%的铜绿假单胞菌菌株对所有测试的抗生素敏感，这一比例在 2008 年降至 47.5%（P<0.05）。在研究期间，观察到环丙沙星和阿米卡星的中位 MIC 值显著增加（P<0.001）；然而，头孢他啶的 MIC 值没有明显变化。
铜绿假单胞菌仍然是一种重要的医院病原体，对抗菌药物具有较高的总体耐药性，耐药水平正在上升。
