Liberman M C
Department of Otology and Laryngology, Harvard Medical School, Boston.
Toxicol Pathol. 1990;18(1 Pt 2):138-48. doi: 10.1177/019262339001800119.
Techniques currently used in the assessment of structural and/or functional damage to the peripheral auditory system are summarized. Two histological approaches are described: one which allows light microscopic evaluation of all structures of the auditory periphery, and a second which concentrates on the sensory cells and their innervation. The latter technique allows electron microscopic analysis of selected regions after a thorough light microscopic survey. Two electrophysiological methods are described as well: a single-fiber approach which provides detailed information about cochlear condition at all frequency locations and a simpler and faster evoked-potential approach which is well suited to screening for cochlear changes. The correlations between structural and functional changes are described using examples from studies of acoustic injury of the inner ear.
本文总结了目前用于评估外周听觉系统结构和/或功能损伤的技术。描述了两种组织学方法：一种可对听觉外周的所有结构进行光学显微镜评估，另一种则专注于感觉细胞及其神经支配。后一种技术在进行全面的光学显微镜检查后，可对选定区域进行电子显微镜分析。还描述了两种电生理方法：一种单纤维方法，可提供所有频率位置的耳蜗状况的详细信息；另一种更简单、快速的诱发电位方法，非常适合用于筛查耳蜗变化。通过内耳声学损伤研究的实例，描述了结构和功能变化之间的相关性。
