Majkut Paul, Böhrsch Verena, Serwa Remigiusz, Gerrits Michael, Hackenberger Christian P R
Institut für Chemie und Biochemie, Freie Universität Berlin, Berlin, Germany.
Methods Mol Biol. 2012;794:241-9. doi: 10.1007/978-1-61779-331-8_15.
Chemoselective reactions are important tools for the modification of peptides and proteins. Thereby the modification is desired to be site specific and bioorthogonal. Here we describe the site-specific modification of azido-proteins via a Staudinger-type phosphite ligation. The reaction was carried out in aqueous system on proteins containing p-azido-phenylalanine in a single position introduced by the amber codon technique. A selective introduction of branched polyethylene scaffolds can be achieved with the application of the methodology reported herein.
化学选择性反应是修饰肽和蛋白质的重要工具。因此，期望修饰具有位点特异性和生物正交性。在此，我们描述了通过施陶丁格型亚磷酸酯连接对叠氮基蛋白质进行位点特异性修饰。该反应在水性体系中对通过琥珀密码子技术在单个位置含有对叠氮基苯丙氨酸的蛋白质进行。应用本文报道的方法可以实现支链聚乙烯支架的选择性引入。
