Fisher George H, Tsesarskaia Mara
Department of Chemistry, Barry University, Miami Shores, FL, USA.
Methods Mol Biol. 2012;794:253-64. doi: 10.1007/978-1-61779-331-8_16.
D-Amino acids are stereoisomers or optical isomers of naturally occurring L-amino acids and thus possess the same chemical structure, but may differ in their biological/physiological properties. Until a half century ago, D-amino acids had been considered to be unnatural substances found only in microorganisms. However, improvements in analytical instruments and methods have revealed that D-amino acids are present in invertebrates and vertebrates, including humans, and that they possess important physiological functions. D-Aspartate (D-Asp) and its methylated form N-methyl-D-aspartate (NMDA) possess neuroendocrine properties in many species. Several methods have been developed for determination of D- and L-enantiomers of amino acids by high performance liquid chromatography (HPLC). We report here improved HPLC methods for the specific determination of D-Asp and NMDA in biological tissues.
D-氨基酸是天然存在的L-氨基酸的立体异构体或旋光异构体，因此具有相同的化学结构，但它们的生物学/生理学特性可能不同。直到半个世纪前，D-氨基酸一直被认为是仅在微生物中发现的非天然物质。然而，分析仪器和方法的改进表明，D-氨基酸存在于包括人类在内的无脊椎动物和脊椎动物中，并且它们具有重要的生理功能。D-天冬氨酸（D-Asp）及其甲基化形式N-甲基-D-天冬氨酸（NMDA）在许多物种中具有神经内分泌特性。已经开发了几种通过高效液相色谱（HPLC）测定氨基酸D-和L-对映体的方法。我们在此报告用于特异性测定生物组织中D-Asp和NMDA的改进的HPLC方法。
