Department of Biochemistry and Molecular Biology, UMDNJ-New Jersey Medical School, Newark, NJ, USA.
Wiley Interdiscip Rev RNA. 2011 Jan-Feb;2(1):22-31. doi: 10.1002/wrna.47. Epub 2010 Sep 20.
Alternative RNA processing mechanisms, including alternative splicing and alternative polyadenylation, are increasingly recognized as important regulators of gene expression. This article will focus on what has recently been described about alternative polyadenylation in development, differentiation, and disease in higher eukaryotes. We will also describe how the evolving global methodologies for examining the cellular transcriptome, both experimental and bioinformatic, are revealing new details about the complex nature of alternative 3(') end formation as well as interactions with other RNA-mediated and RNA processing mechanisms.
替代 RNA 加工机制，包括可变剪接和可变多聚腺苷酸化，越来越被认为是基因表达的重要调控因子。本文将重点介绍在高等真核生物的发育、分化和疾病中最近描述的可变多聚腺苷酸化作用。我们还将描述用于研究细胞转录组的不断发展的全局方法，包括实验和生物信息学方法，如何揭示可变 3(') 末端形成的复杂性质以及与其他 RNA 介导和 RNA 加工机制相互作用的新细节。
