Département de Chimie Moléculaire, UMR CNRS 5250, Université Joseph Fourier, BP 53, 38041 Grenoble Cedex 9, France.
Org Lett. 2011 Oct 21;13(20):5672-5. doi: 10.1021/ol202397e. Epub 2011 Sep 29.
The use of a diazo transfer (DZT) reagent enables clean and efficient conversion of aminated oligodeoxyribonucleotides (ODNs) into their azido counterparts under mild conditions. ODNs bearing an amino tether at the 3', 5', or any internal position could be modified in this manner thus demonstrating the versatility of this reaction. Easy access to such azido-modified ODNs is of great interest for conjugation in particular through copper catalyzed 1,3-dipolar cycloaddition (CuAAC reaction).
重氮转移（DZT）试剂的使用可以在温和条件下将氨基化寡脱氧核苷酸（ODN）干净高效地转化为叠氮化物。带有氨基连接体的 ODN 可以在 3'、5' 位或任何内部位置进行修饰，从而证明了该反应的多功能性。易于获得这种叠氮修饰的 ODN 对于缀合反应特别有意义，尤其是通过铜催化的 1,3-偶极环加成（CuAAC 反应）。
