[军团菌通过质粒DNA进行的转化]

[The transformation of legionellae by plasmid DNA].

作者信息

Marakusha B I, Feoktistova E Iu, Tartakovskiĭ I S, Prozorovskiĭ S V

出版信息

Zh Mikrobiol Epidemiol Immunobiol. 1990 Mar(3):20-3.

PMID:2195809

Abstract

For the first time the possibility of the genetic transformation of L. pneumophila and L. bozemanii strains with the use of purified DNA of plasmids pUC19, pUC4K, pSC101 and RSF1010-pBR322 was shown. The frequency of transformation varied from 5.2 x 10(-6) to 5.8 x 10(-7), depending on the strain used in the experiment and plasmid DNA. In some of the transformants obtained in this investigation plasmid DNA whose molecular weight was similar to that of the plasmid DNA used for transformation was detected. The relatively stable preservation of plasmids pSC101 and RSF1010 in Legionella strains and the loss of plasmids pUC19, pUC4K and pBR322 in 80% of transformants during storage were shown.

摘要

首次证明了利用质粒pUC19、pUC4K、pSC101和RSF1010 - pBR322的纯化DNA对嗜肺军团菌和博兹曼军团菌菌株进行遗传转化的可能性。转化频率从5.2×10⁻⁶到5.8×10⁻⁷不等，这取决于实验中使用的菌株和质粒DNA。在本次研究获得的一些转化体中，检测到分子量与用于转化的质粒DNA相似的质粒DNA。结果表明，质粒pSC101和RSF1010在军团菌菌株中保存相对稳定，而质粒pUC19、pUC4K和pBR322在80%的转化体储存过程中丢失。