Hinton R A, Papathanasiou P, O'Neill H C
Immunology and Stem Cell Laboratory, Research School of Biology, Australian National University, Acton, ACT, AustraliaJohn Curtin School of Medical Research, Australian National University, Acton, ACT, Australia.
Scand J Immunol. 2012 Feb;75(2):168-75. doi: 10.1111/j.1365-3083.2011.02643.x.
Spleen and bone marrow (BM) have been shown to contain the progenitors of a novel dendritic-like antigen-presenting cell type (L-DC). These progenitors are also maintained in both long-term spleen cultures and co-cultures of spleen or BM over the stromal cell line STX3. We examined mouse foetal liver (FL), rich in hematopoietic stem/progenitor cells (HSC/HPC) after embryonic day (E) 12.5, for the presence of L-DC progenitors by testing their capacity to colonize STX3 and produce L-DC. E14.5 FL from wild-type C57BL/6J mice was found to colonize STX3 and produce L-DC for 28 days. By contrast, E14.5 FL from Ikaros Plastic mice gave only short-term production of low numbers of L-DC between 7 and 14 days of co-culture. The transient and weak production of L-DC by FL from Plastic E14.5 mice maps to the loss of self-renewal capacity amongst HSC. L-DC progenitors are, therefore, closely aligned with a subset of self-renewing HSC/HPC in FL.
脾脏和骨髓已被证明含有一种新型树突状样抗原呈递细胞类型（L-DC）的祖细胞。这些祖细胞在长期脾脏培养物以及脾脏或骨髓与基质细胞系STX3的共培养物中也能得以维持。我们检测了胚胎第12.5天（E12.5）后富含造血干细胞/祖细胞（HSC/HPC）的小鼠胎肝（FL），通过测试其在STX3上定植并产生L-DC的能力来检测L-DC祖细胞的存在情况。发现来自野生型C57BL/6J小鼠的E14.5胎肝能够在STX3上定植并产生L-DC达28天。相比之下，来自Ikaros Plastic小鼠的E14.5胎肝在共培养7至14天之间仅能短期产生少量L-DC。来自Plastic E14.5小鼠的胎肝对L-DC的短暂且微弱产生与HSC中自我更新能力的丧失有关。因此，L-DC祖细胞与胎肝中自我更新的HSC/HPC的一个亚群密切相关。
