Laboratory of Experimental Surgical Oncology, Section of Surgical Oncology, Department of Surgery, Erasmus Medical Center, 's Gravendijkwal 230, Rotterdam, The Netherlands.
Eur J Cancer. 2011 Nov;47(17):2576-84. doi: 10.1016/j.ejca.2011.08.016. Epub 2011 Sep 28.
Angiogenesis has been associated with disease progression in many solid tumours, however the statement that tumours need angiogenesis to grow, invade and metastasise seems no longer applicable to all tumours or to all tumour subtypes. Prognostic studies in pancreatic cancer are conflicting. In fact, pancreatic cancer has been suggested an example of a tumour in which angiogenesis is less essential for tumour progression. The aim of the present study was therefore to measure angiogenesis in two anatomically closely related however prognostically different types of pancreatic cancer, pancreatic head and periampullary cancer, and investigate its relation with outcome. Vessels were stained by CD31 on original paraffin embedded tissue from 206 patients with microscopic radical resection (R0) of pancreatic head (n=98) or periampullary cancer (n=108). Angiogenesis was quantified by microvessel density (MVD) and measured by computerised image analysis of three randomly selected fields and investigated for associations with recurrence free survival (RFS), cancer specific survival (CSS), overall survival (OS) and conventional prognostic factors. MVD was heterogeneous both between and within tumours. A higher MVD was observed in periampullary cancers compared with pancreatic head cancers (p<.01). Furthermore, MVD was associated with lymph node involvement in pancreatic head (p=.014), but not in periampullary cancer (p=.55). Interestingly, MVD was not associated with RFS, CSS or with OS. In conclusion, angiogenesis is higher in periampullary cancer and although associated with nodal involvement in pancreatic head cancer, pancreatic cancer prognosis seems indeed angiogenesis independent.
血管生成与许多实体瘤的疾病进展有关，然而，肿瘤需要血管生成才能生长、侵袭和转移的说法似乎不再适用于所有肿瘤或所有肿瘤亚型。胰腺癌的预后研究存在矛盾。事实上，已经有人提出，胰腺癌是一种血管生成对肿瘤进展不太重要的肿瘤的例子。因此，本研究的目的是测量两种解剖上密切相关但预后不同的胰腺癌（胰头癌和胰周癌）中的血管生成，并研究其与预后的关系。对 206 例接受显微镜下根治性切除（R0）的胰头癌（n=98）或胰周癌（n=108）患者的原始石蜡包埋组织进行 CD31 染色，以检测血管生成。通过计算机图像分析三个随机选择的视野来量化微血管密度（MVD），并对其进行测量，并将其与无复发生存率（RFS）、癌症特异性生存率（CSS）、总生存率（OS）和传统预后因素进行关联分析。MVD 在肿瘤之间和肿瘤内均存在异质性。胰周癌的 MVD 高于胰头癌（p<.01）。此外，MVD 与胰头癌的淋巴结受累有关（p=.014），但与胰周癌无关（p=.55）。有趣的是，MVD 与 RFS、CSS 或 OS 无关。总之，胰周癌中的血管生成较高，尽管与胰头癌中的淋巴结受累有关，但胰腺癌的预后似乎确实与血管生成无关。
