利用茶籽油皂脚和碳酸二乙酯酶法合成脂肪酸乙酯。
Enzymatic synthesis of fatty acid ethyl esters by utilizing camellia oil soapstocks and diethyl carbonate.
机构信息
State Key Laboratory of Bioreactor Engineering, Department of Bioengineerng, East China University of Science and Technology, Shanghai 200237, China.
出版信息
Bioresour Technol. 2011 Nov;102(22):10173-9. doi: 10.1016/j.biortech.2011.09.004. Epub 2011 Sep 10.
This study was reported on a novel process for fatty acid ethyl esters preparation by transesterification and esterification from renewable low-cost feedstock camellia oil soapstocks and friendly acyl acceptor diethyl carbonate. The main components of product were 83.9% ethyl oleate, 8.9% ethyl palmitate, 4.7% ethyl linoleate and 2.1% ethyl stearate, which could be used as eco-friendly renewable resources or additives of industrial solvent and fossil fuel. The effects of molar ratio of diethyl carbonate to soapstocks oil, lipases, organic solvent, reaction temperature and time were investigated, and process conditions were optimized. The yield was up to 98.4% in solvent-free system with molar ratio of diethyl carbonate to soapstocks oil 3:1 and 5% Novozym 435 (based on the weight of soapstocks oil) at 50 °C and 180 rpm for 24 h. Moreover, there was no obvious loss in the yield after lipases were reused for 10 batches without treatment under optimized conditions.
本研究报道了一种从可再生的低成本原料茶皂素和友好的酰基接受体碳酸二乙酯通过酯交换和酯化制备脂肪酸乙酯的新工艺。产物的主要成分是 83.9%的油酸乙酯、8.9%的棕榈酸乙酯、4.7%的亚油酸乙酯和 2.1%的硬脂酸乙酯，可作为环保可再生资源或工业溶剂和化石燃料的添加剂。考察了碳酸二乙酯与皂素油的摩尔比、脂肪酶、有机溶剂、反应温度和时间的影响，并对工艺条件进行了优化。在无溶剂体系中，当碳酸二乙酯与皂素油的摩尔比为 3:1，用量为 5%的 Novozym 435（基于皂素油的重量），在 50°C 和 180rpm 下反应 24 小时，产率可达 98.4%。此外，在优化条件下，脂肪酶在未经处理的情况下重复使用 10 批后，产率没有明显下降。
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