Deng Li, Tan Tian-Wei, Wang Fang
Beijing Bioprocess Key Lab, Beijing University of Chemical Technology, Department of Bioengineering, Beijing 100029, China.
Sheng Wu Gong Cheng Xue Bao. 2003 Jan;19(1):97-101.
Biodiesel, an alternative diesel fuel, fatty acid alkyl ester, is made from renewable biological sources such as vegetable oils and animal fats. Two processes for biodiesel synthesis, enzymatic lipase catalytic esterification from fatty acid and transesterification from oils and fats, was investigated. The effects of various lipases, enzyme amount and purity, solvent, water absorbent, inhibition of short chains alcohol, specificity of substrate, molar ratio of substrate on esterification were studied in detail. The esterification degree with the optimal parameter and process can reach up to 92%. The purity of biodiesel obtained by separation and purification is up to 98%, and the half-life of the immobilized lipase for the esterification process can be up to 360hr, Moreover, the preliminary studies of the transesterification including the amount of methanol and mode of adding methanol into reaction system were made. The transesterification degree with adding methanol stepwise can reach 83%.
生物柴油是一种替代柴油燃料，即脂肪酸烷基酯，由植物油和动物脂肪等可再生生物资源制成。研究了生物柴油合成的两种方法，即脂肪酸的酶促脂肪酶催化酯化和油脂的酯交换反应。详细研究了各种脂肪酶、酶量和纯度、溶剂、吸水剂、短链醇的抑制作用、底物特异性、底物摩尔比对酯化反应的影响。在最佳参数和工艺条件下，酯化度可达92%。通过分离纯化得到的生物柴油纯度可达98%，固定化脂肪酶在酯化过程中的半衰期可达360小时。此外，还对酯交换反应进行了初步研究，包括甲醇用量和甲醇加入反应体系的方式。逐步加入甲醇时，酯交换度可达83%。
