Department of Dental Biomaterials, School of Dentistry, Brain Korea 21 Project, Chonbuk National University, Jeonju, South Korea.
Colloids Surf B Biointerfaces. 2012 Jan 1;89:101-7. doi: 10.1016/j.colsurfb.2011.09.001. Epub 2011 Sep 10.
The dispersing power of surfactant-modified multiwalled carbon nanotubes (MWCNTs) and their effect on the antibacterial activity were examined. The MWCNTs were modified using a dioctyl sodium sulfosuccinate (AOT) surfactant. UV-vis spectroscopy and transmission electron microscopy (TEM) were used to characterize the dispersion of MWCNTs in the aqueous phase. Fourier transform infrared spectroscopy confirmed the results of UV-vis spectroscopy and TEM, indicating that the AOT molecules had been adsorbed successfully onto the MWCNT surface. The highly dispersed AOT-modified MWCNTs showed strong antibacterial activity to Streptococcus mutans. The fluorescence images showed that the AOT-modified MWCNTs were capable of capturing bacteria and forming cell aggregates as well as killing them. The optical density growth curves and colony-forming units assays confirmed that the antibacterial activity of the AOT-modified MWCNTs was concentration-dependent and treatment time-dependent. This finding might be useful for applications of AOT-modified MWCNTs as an antibacterial agent to eliminate pathogens from a biocontaminated water phase.
研究了表面活性剂修饰的多壁碳纳米管（MWCNTs）的分散能力及其对抑菌活性的影响。采用二辛基琥珀酸磺酸钠（AOT）表面活性剂对 MWCNTs 进行修饰。利用紫外可见分光光度法和透射电子显微镜（TEM）对 MWCNTs 在水相中的分散情况进行了表征。傅里叶变换红外光谱（FTIR）证实了紫外可见分光光度法和 TEM 的结果，表明 AOT 分子已成功吸附到 MWCNT 表面。高度分散的 AOT 修饰 MWCNTs 对变形链球菌表现出很强的抑菌活性。荧光图像显示，AOT 修饰的 MWCNTs 能够捕获细菌并形成细胞聚集，同时杀死细菌。光密度生长曲线和集落形成单位检测证实，AOT 修饰的 MWCNTs 的抑菌活性与浓度和处理时间有关。这一发现可能有助于将 AOT 修饰的 MWCNTs 作为一种抗菌剂应用于消除生物污染水相中病原体的研究。
