Du Xiaoping, Choi Chul-Hee, Chen Kejian, Cheng Weihua, Floyd Robert A, Kopke Richard D
Hough Ear Institute, Oklahoma City, OK 73112, USA.
Int J Otolaryngol. 2011;2011:612690. doi: 10.1155/2011/612690. Epub 2011 Sep 25.
Objective. Inhibition of inflammation and free radical formation in the cochlea may be involved in antioxidant treatment in acute acoustic trauma. Procedure. Chinchilla were exposed to 105 dB sound pressure level octave band noise for 6 hours. One group of chinchilla was treated with antioxidants after noise exposure. Auditory brainstem responses, outer hair cell counts, and immunohistochemical analyses of biomarkers in the cochlea were conducted. Results. The antioxidant treatment significantly reduced hearing threshold shifts, outer hair cell loss, numbers of CD45(+) cells, as well as 4-hydroxy-2-nonenal and nitrotyrosine formation in the cochlea. Conclusion. Antioxidant treatment may provide protection to sensory cells by inhibiting formation of reactive oxygen and nitrogen products and migration of mononuclear phagocytes in the cochlea. The present study provides further evidence of effectiveness of antioxidant treatment in reducing permanent hearing loss.
目的。抑制耳蜗中的炎症和自由基形成可能与急性声创伤的抗氧化治疗有关。方法。将灰鼠暴露于105分贝声压级倍频程带噪声中6小时。一组灰鼠在噪声暴露后接受抗氧化剂治疗。进行了听觉脑干反应、外毛细胞计数以及耳蜗中生物标志物的免疫组织化学分析。结果。抗氧化剂治疗显著降低了听力阈值偏移、外毛细胞损失、CD45(+)细胞数量，以及耳蜗中4-羟基-2-壬烯醛和硝基酪氨酸的形成。结论。抗氧化剂治疗可能通过抑制耳蜗中活性氧和氮产物的形成以及单核吞噬细胞的迁移来保护感觉细胞。本研究为抗氧化剂治疗在减少永久性听力损失方面的有效性提供了进一步证据。
