Faculty of Chemistry, Jagiellonian University, Krakow, Poland.
Analyst. 2011 Nov 21;136(22):4704-9. doi: 10.1039/c1an15598a. Epub 2011 Sep 30.
The potential use of surface Raman enhanced spectroscopy (SERS) for confirmatory identification and the semi-quantitative analysis of selected tricyclic antidepressants (TCAs) is examined utilizing a conventional silver colloid. Raman and SERS spectra of aqueous solutions of imipramine (Imi) and its metabolite, desipramine (Des), were recorded as the function of concentration using NIR excitation. A good linear correlation is observed for the dependence of the SERS signal at 684 cm(-1) (R(2) = 0.9997) on Imi concentration over the range of 0.75-7.5 μM. The limit of detection of imipramine in the silver colloidal solution is 0.98 μM. SERS spectra of Imi and Des were also recorded for blood plasma samples without prior purification as well as after the use of standard solid phase extraction. All spectra show the characteristic spectral profile of the molecules and moreover, stronger signal enhancement is observed for Imi in the "raw" samples as opposed to Imi extracted from a biological matrix.
表面增强拉曼光谱（SERS）在确证鉴定和半定量分析选定的三环抗抑郁药（TCAs）方面的潜在应用，利用常规的银胶体进行了研究。记录了水溶液中丙咪嗪（Imi）及其代谢物去甲丙咪嗪（Des）的拉曼和 SERS 光谱，作为浓度的函数，使用近红外激发。在 0.75-7.5 μM 的范围内，观察到 SERS 信号在 684 cm(-1)（R(2) = 0.9997）处对 Imi 浓度的依赖性呈良好的线性关系。在银胶体溶液中丙咪嗪的检测限为 0.98 μM。还记录了未经预先净化以及使用标准固相萃取后血浆样品中的 Imi 和 Des 的 SERS 光谱。所有光谱均显示分子的特征光谱轮廓，此外，与从生物基质中提取的 Imi 相比，在“原始”样品中观察到 Imi 的信号增强更强。
