Trace Analysis and Biosensor Research Center, Prince of Songkla University, Hat Yai, Songkhla, Thailand.
J Environ Sci Health A Tox Hazard Subst Environ Eng. 2011;46(13):1450-60. doi: 10.1080/10934529.2011.609022.
This work presents the results of the use of flow injection surface plasmon resonance and impedimetric affinity biosensors for detecting and enumerating total bacteria based on the binding between E. coli and Con A, immobilized on a modified gold electrode. The single analysis time for both techniques was less than 20 min. Dissociation between the immobilized Con A and the E. coli using 200 mM of glucose in HCl at pH of 2.00 enabling the sensor to be reused for between 29-35 times. Impedimetric detection provided a much lower limit of detection (12 CFU mL(-1)) than the surface plasmon resonance method (6.1 × 10(7) CFU mL(-1)). Using the impedimetric system, real sample analysis was performed and the results were compared to the plate count agar method. Cell concentrations obtained by the biosensor were only slightly different from the result obtained from the plate count agar. The proposed system offers a rapid and useful tool for screening detection and enumeration of total bacteria.
本工作展示了使用流动注射表面等离子体共振和阻抗亲和生物传感器基于 E. coli 与 Con A 之间的结合来检测和计数总细菌的结果，Con A 与 E. coli 固定在修饰的金电极上。两种技术的单个分析时间都小于 20 分钟。使用 200mM 的 HCl 中的葡萄糖在 pH 值为 2.00 时将固定的 Con A 与 E. coli 解吸，使传感器能够重复使用 29-35 次。与表面等离子体共振方法相比（6.1×10(7)CFU mL(-1)），阻抗检测法的检测限要低得多（12CFU mL(-1)）。使用阻抗系统进行了实际样品分析，并将结果与平板计数琼脂方法进行了比较。生物传感器获得的细胞浓度与平板计数琼脂法获得的结果仅略有不同。该系统为总细菌的筛选检测和计数提供了一种快速有用的工具。
