椰子（可可椰子）种子的分子鉴定。
Molecular identification of Lodoicea maldivica (coco de mer) seeds.
机构信息
Government Laboratory Hong Kong, 7/F Ho Man Tin Government Offices, 88 Chung Hau Street, Kowloon, Hong Kong SAR, China.
出版信息
Chin Med. 2011 Sep 30;6(1):34. doi: 10.1186/1749-8546-6-34.
BACKGROUND
The edible endosperm of Lodoicea maldivica with the common name of coco de mer is used in Chinese medicine for treating cough. Native to Seychelles, Lodoicea maldivica seeds have commanded high prices for centuries due to its scarcity. This study aims to develop a molecular identification method for the authentication of Lodoicea maldivica seeds.
METHODS
DNA was extracted from the sample. Two polymerase chain reaction (PCR) systems were developed to amplify a region of the chloroplast DNA and the nuclear phosphoribulokinase (PRK) region specific to Lodoicea maldivica respectively. DNA sequence of a sample was determined and compared with that of the Lodoicea maldivica reference material.
RESULTS
The PRK gene of Lodoicea maldivica was successfully amplified and sequenced for identification.
CONCLUSION
A new molecular method for the identification of Lodoicea maldivica seeds in fresh, frozen or dried forms was developed.
背景
可可椰子的可食用胚乳在中文医学中用于治疗咳嗽。原产于塞舌尔的可可椰子种子由于其稀缺性，几个世纪以来一直价格高昂。本研究旨在开发一种分子鉴定方法，用于鉴定可可椰子种子。
方法
从样品中提取 DNA。开发了两个聚合酶链反应 (PCR) 系统，分别扩增叶绿体 DNA 和核磷酸核糖激酶 (PRK) 区域的特定可可椰子区域。确定了样本的 DNA 序列，并与可可椰子参考材料的 DNA 序列进行了比较。
结果
成功扩增并测序可可椰子的 PRK 基因，以进行鉴定。
结论
开发了一种新的分子方法，可用于鉴定新鲜、冷冻或干燥形式的可可椰子种子。
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