Radiation Safety Department, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, NIH, Rocky Mountain Laboratories , Hamilton, MT 59840, USA.
Int J Radiat Biol. 2011 Oct;87(10):1039-44. doi: 10.3109/09553002.2011.598210.
To evaluate an innovative photo-fluorescent film as a routine dosimetric tool during (60)Co irradiations at a high containment biological research laboratory, and to investigate whether manufacturer-provided chamber exposure rates can be used to accurately administer a prescribed dose to biological specimens.
Photo-fluorescent, lithium fluoride film dosimeters and National Institutes of Standards and Technology (NIST) transfer dosimeters were co-located in a self-shielded (60)Co irradiator and exposed to γ-radiation with doses ranging from 5-85 kGy. Film dose-response relationships were developed for varying temperatures simulating conditions present when irradiating infectious biological specimens. Dose measurement results from NIST transfer dosimeters were compared to doses predicted using manufacturer-provided irradiator chamber exposure rates.
The film dosimeter exhibited a photo-fluorescent response signal that was consistent and nearly linear in relationship to γ-radiation exposure over a wide dose range. The dosimeter response also showed negligible effects from dose fractionization and humidity. Significant disparities existed between manufacturer-provided chamber exposure rates and actual doses administered.
This study demonstrates the merit of utilizing dosimetric tools to validate the process of exposing dangerous and exotic biological agents to γ-radiation at high containment laboratories. The film dosimeter used in this study can be utilized to eliminate potential for improperly administering γ-radiation doses.
评估一种创新的光致荧光薄膜作为高隔离生物研究实验室中（60）Co 辐照的常规剂量学工具，并研究制造商提供的腔室照射率是否可用于准确向生物标本施予规定剂量。
光致荧光氟化锂薄膜剂量计和美国国家标准与技术研究院（NIST）传递剂量计被放置在一个自屏蔽（60）Co 辐照器中，并用剂量范围为 5-85 kGy 的 γ 射线进行照射。为模拟辐照传染性生物标本时的条件，对不同温度下的薄膜剂量响应关系进行了研究。使用制造商提供的辐照腔室照射率比较了 NIST 传递剂量计的剂量测量结果和预测的剂量。
薄膜剂量计的光致荧光响应信号在宽剂量范围内与 γ 射线暴露呈一致且近乎线性的关系。该剂量计的响应还显示出剂量分割和湿度的影响可以忽略不计。制造商提供的腔室照射率与实际施予的剂量之间存在显著差异。
本研究证明了在高隔离实验室中利用剂量学工具验证暴露于危险和奇异生物制剂的 γ 辐射过程的优点。本研究中使用的薄膜剂量计可用于消除不正确施予 γ 辐射剂量的潜在风险。
