木薯浆同步生产乙醇和氢气的生物炼制工艺
A consolidated bio-processing of ethanol from cassava pulp accompanied by hydrogen production.
机构信息
School of Bioscience and Bioengineering, South China University of Technology, Guangzhou Higher Education Mega Centre, Panyu, Guangzhou 510006, People's Republic of China.
出版信息
Bioresour Technol. 2011 Nov;102(22):10471-9. doi: 10.1016/j.biortech.2011.08.134. Epub 2011 Sep 10.
A biphasic fermentation approach was undertaken for the production of ethanol and hydrogen from cassava pulp. The glucose generated by co-culture of Clostridium thermocellum and Thermoanaerobacterium aotearoense was 13.65±0.45 g L(-1), which was 1.75 and 1.17-fold greater than that produced by mono-cultures of C. thermocellum and T. aotearoense, respectively. The accumulated glucose could be utilised rapidly by subsequently inoculated Saccharomyces cerevisiae. An inoculum ratio of 1:1, a thermophilic fermentation of 84 h, and a pulp concentration of 4% proved optimal for ethanol production, fermentation efficiency, and productivity. With these conditions, the ethanol level reached 8.83±0.31 g L(-1) with a fermentation efficiency of 64.95±2.71%. Hydrogen production of 4.06 mmol by the co-culture system was 1.54 and 2.09-fold greater than that produced by mono-cultures of C. thermocellum and T. aotearoense, respectively. This sequential co-culture approach provided a consolidated bio-processing means to produce ethanol and hydrogen from cassava pulp.
采用两段式发酵法从木薯浆中生产乙醇和氢气。协同培养的嗜热梭菌（Clostridium thermocellum）和热厌氧菌（Thermoanaerobacterium aotearoense）产生的葡萄糖为 13.65±0.45 g L(-1)，分别比单独培养的嗜热梭菌和热厌氧菌高 1.75 倍和 1.17 倍。随后接种的酿酒酵母（Saccharomyces cerevisiae）可以快速利用积累的葡萄糖。最佳的乙醇生产条件为：接种比例 1:1、嗜热发酵 84 h 和浆浓度 4%。在这些条件下，乙醇水平达到 8.83±0.31 g L(-1)，发酵效率为 64.95±2.71%。协同培养系统产生的氢气为 4.06 mmol，分别比单独培养的嗜热梭菌和热厌氧菌高 1.54 倍和 2.09 倍。这种顺序共培养方法为从木薯浆中生产乙醇和氢气提供了一种综合的生物加工手段。
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