Key Laboratory of Analytical Chemistry for Biology and Medicine (Wuhan University), Ministry of Education, Wuhan 430072, China.
Talanta. 2011 Oct 15;85(5):2581-6. doi: 10.1016/j.talanta.2011.08.021. Epub 2011 Aug 16.
In this work, a two-step liquid-phase microextraction (LPME) method was presented for the extraction of phenols in environmental water samples. Firstly, the polar phenol in water samples (donor phase) was transferred to 1-octanol (extraction mesophase) by magnetic stirring-assisted LPME. Subsequently, target analytes in the 1-octanol was back extracted into 0.1 mol/L sodium hydroxide solution (acceptor phase) by vortex-assisted LPME. By combination of the two-step LPME, the enrichment factors were multiplied. The main features of this two-step LPME for phenols lie in the following aspects. Firstly, the extraction can be accomplished within relatively short time (ca. 20 min). Secondly, it was compatible with HPLC analysis, avoiding derivatization step that is generally necessary for GC analysis. Thirdly, high enrichment factors (296-954 fold) could be obtained for these analytes. Under the optimized conditions, the linearities were 10-1000, 1-500, 1-500, 5-500 and 1-500 ng/mL for different phenols with all regression coefficients higher than 0.9985. The limits of detection were in the range from 0.3 to 3.0 ng/mL for these analytes. Intra-and inter-day relative standard deviations were below 7.6%, indicating a good precision of the proposed method.
在这项工作中，提出了一种两步液相微萃取（LPME）方法，用于萃取环境水样中的酚类物质。首先，通过磁搅拌辅助 LPME 将水样品中的极性酚（供体相）转移到 1-辛醇（萃取介质相）中。随后，通过涡旋辅助 LPME 将目标分析物从 1-辛醇中反萃取到 0.1mol/L 氢氧化钠溶液（受体相）中。通过两步 LPME 的结合，富集因子相乘。这种两步 LPME 萃取酚类物质的主要特点如下。首先，萃取可以在相对较短的时间内完成（约 20 分钟）。其次，它与 HPLC 分析兼容，避免了通常 GC 分析所必需的衍生化步骤。第三，这些分析物可以获得高的富集因子（296-954 倍）。在优化条件下，不同酚类物质的线性范围为 10-1000、1-500、1-500、5-500 和 1-500ng/mL，所有回归系数均高于 0.9985。这些分析物的检出限在 0.3-3.0ng/mL 范围内。日内和日间相对标准偏差均低于 7.6%，表明该方法具有良好的精密度。
