Institute of Analytical Instruments, School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou, Guangdong 510006, PR China.
Talanta. 2011 Oct 15;85(5):2614-9. doi: 10.1016/j.talanta.2011.08.029. Epub 2011 Aug 19.
A microfluidic chip manufactured from glass substrate and indium tin oxide (ITO) coated glass use for contactless conductivity detection was developed. The detecting electrodes were fabricated by screen-printing and chemical etching methods using an ITO-coated glass wafer. Then, the glass substrate containing separation channels was bonded with the bare side of the processed ITO-coated glass, thus producing an electrophoresis chip integrated with contactless conductivity detector. The prepared microchip displayed considerable stability and reproducibility. Sensitive response was obtained at optimal conditions (including the gap between electrodes, excitation frequency, and excitation voltage). The feasibility of this microfluidic device was examined by detection of inorganic ions, and further demonstrated by the quantification of aminopyrine and caffeine in a compound pharmaceutical. The two ingredients can be completely separated within 1 min. The detection limits were 8 μg mL(-1) and 3 μg mL(-1), respectively; with the correlation coefficient of 0.996-0.998 in the linear range from 10 μg mL(-1) to 800 μg mL(-1). The results have showed that the present method is sensitive, reliable and fast.
一种由玻璃基底和铟锡氧化物（ITO）涂层玻璃制造的用于非接触式电导检测的微流控芯片已经开发出来。检测电极通过丝网印刷和化学蚀刻方法使用 ITO 涂层玻璃晶片制造。然后，含有分离通道的玻璃基底与处理过的 ITO 涂层玻璃的裸露侧键合，从而产生与非接触式电导检测器集成的电泳芯片。所制备的微芯片显示出相当的稳定性和重现性。在最佳条件下（包括电极之间的间隙、激励频率和激励电压）获得了灵敏的响应。通过检测无机离子检验了这种微流控器件的可行性，并通过对复方药物中氨基比林和咖啡因的定量进一步证明。两种成分可以在 1 分钟内完全分离。检测限分别为 8 μg mL(-1)和 3 μg mL(-1)；在线性范围从 10 μg mL(-1)到 800 μg mL(-1)内，相关系数为 0.996-0.998。结果表明，该方法灵敏、可靠、快速。
