Oral Translational Research, Institute of Oral Biology, Center of Dental Medicine, University of Zurich, Zurich, Switzerland.
Cytokine. 2011 Dec;56(3):656-61. doi: 10.1016/j.cyto.2011.08.039. Epub 2011 Oct 1.
Doxycycline is an antibiotic used in the treatment of a variety of inflammatory conditions, including periodontitis. Apart from its antimicrobial properties, this drug also has independent anti-inflammatory effects at sub-antimicrobial doses. The present study aimed to investigate the effects of low-doses of doxycycline (LDD) on cytokine production by human monocytic cells challenged with the periodontal pathogen Aggregatibacter actinomycetemcomitans, for up to 6 h. The simultaneous regulation of 12 cytokines were measured by a Human Cytokine Array Kit. To validate the array findings, selected cytokines were also measured by enzyme-linked immunosorbant assay (ELISA). A. actinomycetemcomitans stimulated the production of tumor necrosis factor (TNF)-α, interleukin (IL)-1α, IL-1β, IL-6 and IL-8 by the cells after 6 h of challenge, and doxycycline significantly inhibited this effect. The kinetics of this regulation demonstrated an early (within 2 h) and significant (P<0.05) inhibition of pro-inflammatory cytokines, with a mild (0.5-fold) up-regulation of the anti-inflammatory cytokine IL-10. The results indicate that LDD acts as an anti-inflammatory agent in human monocytic cells stimulated with A. actinomycetemcomitans. This model provides clear evidence that some of the clinically proven benefits of LDD may be related to its ability to regulate inflammatory mediator release by monocytic cells. This property may contribute to the clinically proven benefits of this antibiotic as an adjunctive treatment for periodontitis.
强力霉素是一种抗生素，用于治疗多种炎症性疾病，包括牙周炎。除了其抗菌特性外，这种药物在亚抗菌剂量下也具有独立的抗炎作用。本研究旨在探讨低剂量强力霉素（LDD）对人单核细胞在牙周病病原体伴放线放线杆菌刺激下产生细胞因子的影响，刺激时间长达 6 小时。通过人细胞因子阵列试剂盒同时测量 12 种细胞因子的表达。为了验证芯片的结果，还通过酶联免疫吸附试验（ELISA）测量了选定的细胞因子。经过 6 小时的刺激，伴放线放线杆菌刺激细胞产生肿瘤坏死因子（TNF）-α、白细胞介素（IL）-1α、IL-1β、IL-6 和 IL-8，强力霉素显著抑制了这种作用。这种调节的动力学表现出早期（2 小时内）和显著（P<0.05）的促炎细胞因子抑制作用，抗炎细胞因子 IL-10 轻度（0.5 倍）上调。结果表明，LDD 作为伴放线放线杆菌刺激人单核细胞的抗炎剂。该模型提供了明确的证据，表明 LDD 的一些临床证实的益处可能与其调节单核细胞炎症介质释放的能力有关。这种特性可能有助于该抗生素作为牙周炎辅助治疗的临床益处。
