Department of Orthopaedic Surgery, Graduate School of Comprehensive Human Sciences, University of Tsukuba, Tsukuba, Ibaraki, Japan.
Ultrasound Med Biol. 2011 Dec;37(12):2028-36. doi: 10.1016/j.ultrasmedbio.2011.08.010. Epub 2011 Oct 2.
Previously, we successfully applied a new method composed of drilling, bone marrow transplantation (BMT), external fixation and low-intensity pulsed ultrasound (LIPUS) for the clinical treatment of Kienböck's disease. The purpose of this study was to investigate whether bone regeneration can be induced by LIPUS and/or multiple drilling and/or BMT within a severely necrotic small-bone rabbit model. Eighteen rabbits were divided into three groups (BMT, drilling and control) and LIPUS stimulation was introduced daily for 8 weeks post-transplantation. Next, 12 additional rabbits were produced for the BMT group and LIPUS stimulation was introduced daily for 4 and 12 weeks (n = 6 for each). Histopathologically, new bone formations were rarely observed in the drilling and control groups. In the BMT group, the mineralizing surface areas of LIPUS(+) showed a significant increase compared with LIPUS(-) for 8 weeks. LIPUS treatment alone did not accelerate the revitalization of necrotic bones. However, LIPUS combined with BMT tended to promote new bone formation.
先前，我们成功地应用了一种新方法，包括钻孔、骨髓移植（BMT）、外固定和低强度脉冲超声（LIPUS），用于治疗 Kienböck 病的临床治疗。本研究的目的是研究在严重坏死的小型兔模型中，LIPUS 和/或多次钻孔和/或 BMT 是否可以诱导骨再生。18 只兔子被分为三组（BMT、钻孔和对照组），并在移植后每天进行 LIPUS 刺激 8 周。然后，另外 12 只兔子被用于 BMT 组，每天进行 LIPUS 刺激 4 周和 12 周（每组 6 只）。组织病理学观察结果显示，在钻孔和对照组中很少观察到新骨形成。在 BMT 组中，LIPUS(+) 的矿化面积与 LIPUS(-) 相比，在 8 周时显著增加。单独使用 LIPUS 并不能加速坏死骨的复活。然而，LIPUS 联合 BMT 倾向于促进新骨形成。
