Ishihara Yuri, Ueki Koichiro, Sotobori Megumi, Marukawa Kohei, Moroi Akinori
Department of Oral and Maxillofacial Surgery, Division of Medicine, Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi, 1110 Shimokato, Chuo-shi, Yamanashi 409-3898, Japan.
Department of Oral and Maxillofacial Surgery, Division of Medicine, Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi, 1110 Shimokato, Chuo-shi, Yamanashi 409-3898, Japan.
J Craniomaxillofac Surg. 2014 Apr;42(3):185-93. doi: 10.1016/j.jcms.2013.05.002. Epub 2013 Aug 27.
To compare bone regeneration between local implantation of statin and low-intensity pulsed ultrasound (LIPUS), and the combination of statin with LIPUS in rabbit nasal bone using histological and immunohistochemical methods.
Thirty-two adult male Japanese white rabbits (age: 12-16 weeks, weight: 2.5-3.0 kg) were used in this study. Two bone circular defects (5 mm in diameter) per rabbit were created in the nasal bone while preserving the nasal membrane. The two defects in each rabbit were filled with 2.5 mg/ml simvastatin in 0.2 ml water with an atelocollagen sponge (ACS) and ACS alone respectively. Sixteen rabbits (32 sides) received the LIPUS application; the remaining 16 rabbits (32 sides) did not. Therefore, the subjects composed of 4 groups, namely, (1) LIPUS + ACS + simvastatin (the LAS group), (2) LIPUS + ACS (the LA group), (3) ACS + simvastatin (the AS group) and (4) ACS alone (the A group). Four animals were killed in each period, at 1, 2, 4 and 8 weeks postoperative. The parts that had been operated on were removed and prepared for histological assessment. The expression of BMP-2 and the bone area ratio were evaluated using histological and immunohistochemical methods.
Bone square in the LAS group was significantly larger than that in the AS group after 1 (P < 0.0001) and 2 week (P = 0.0113). The bone square in the LA group was significantly larger than that in the A group after 1 (P < 0.0001) and 2 weeks (P = 0.0090). However, there was no significant difference between the LAS and LA groups. In the number of cells that stained positive for BMP-2, the LAS group was significantly larger than that in the AS group after 1 (P < 0.0001) and 2 weeks (P = 0.0113).
This study suggests that bone regeneration can be promoted by LIPUS alone and statin alone, respectively. However the combination use of LIPUS with statin does not differ from LIPUS alone or statin alone.
采用组织学和免疫组织化学方法，比较他汀类药物局部植入、低强度脉冲超声（LIPUS）以及他汀类药物与LIPUS联合应用对兔鼻骨骨再生的影响。
本研究选用32只成年雄性日本白兔（年龄：12 - 16周，体重：2.5 - 3.0 kg）。在每只兔的鼻骨上制作两个圆形骨缺损（直径5 mm），同时保留鼻黏膜。每只兔的两个缺损分别用含2.5 mg/ml辛伐他汀的0.2 ml水与去端胶原蛋白海绵（ACS）填充以及仅用ACS填充。16只兔（32侧）接受LIPUS治疗；其余16只兔（32侧）未接受。因此，实验对象分为4组，即（1）LIPUS + ACS + 辛伐他汀组（LAS组），（2）LIPUS + ACS组（LA组），（3）ACS + 辛伐他汀组（AS组）和（4）仅ACS组（A组）。术后1、2、4和8周，每个时间段处死4只动物。取出手术部位进行组织学评估。采用组织学和免疫组织化学方法评估骨形态发生蛋白-2（BMP - 2）的表达及骨面积比。
术后1周（P < 0.0001）和2周（P = 0.0113），LAS组的骨面积显著大于AS组。术后1周（P < 0.0001）和2周（P = 0.0090），LA组的骨面积显著大于A组。然而，LAS组和LA组之间无显著差异。在BMP - 2染色阳性细胞数量方面，术后1周（P < 0.0001）和2周（P = 0.0113），LAS组显著多于AS组。
本研究表明，单独使用LIPUS和单独使用他汀类药物均可促进骨再生。然而，LIPUS与他汀类药物联合使用与单独使用LIPUS或单独使用他汀类药物相比并无差异。
