Department of Veterinary Clinical Medicine, Iwate University, Morioka 020-8550, Japan.
Res Vet Sci. 2012 Aug;93(1):378-80. doi: 10.1016/j.rvsc.2011.07.010. Epub 2011 Sep 29.
A colorimetric assay used to quantify the non-ionic contrast medium iodixanol in sera was validated and compared with high-performance liquid chromatography. The application of this assay to estimate glomerular filtration rates (GFR) in cattle was examined. Serum iodixanol was de-iodinated by alkaline hydrolysis and the amount of released iodine was subsequently determined using a ceric arsenite method. There was a close correlation between the two methods using identical specimens. In clinically healthy cattle with different body weights, the reference value (166.3-178.8 mL/min/m(2)) based on body surface area was fairly stable as compared with that (2.13-3.63 mL/min/kg) based on body weight. Based on GFR data in healthy and renal-impaired cattle, when the GFR decreased to more than 60% of the reference value, serum urea nitrogen and creatinine concentrations increased. The colorimetric assay is a simple method for the estimation of GFR in cattle and requires no expensive equipment.
一种用于定量检测血清中非离子型造影剂碘克沙醇的比色法已被验证，并与高效液相色谱法进行了比较。该方法在评估牛肾小球滤过率（GFR）中的应用进行了检验。血清中的碘克沙醇经碱性水解脱碘，随后使用砷酸铈法测定释放的碘量。使用相同的标本时，两种方法具有密切的相关性。在体重不同但临床健康的牛中，基于体表面积的参考值（166.3-178.8 mL/min/m²）与基于体重的参考值（2.13-3.63 mL/min/kg）相比相当稳定。基于健康和肾功能受损牛的 GFR 数据，当 GFR 下降到参考值的 60%以上时，血清尿素氮和肌酐浓度升高。该比色法是一种简单的牛 GFR 估算方法，不需要昂贵的设备。
