Faculty of Science, Tokyo University of Science, Tokyo, Japan.
J Microbiol Methods. 2011 Dec;87(3):316-9. doi: 10.1016/j.mimet.2011.09.006. Epub 2011 Sep 21.
We describe a trajectory analysis of diatom cell locomotion by combining a micro chamber and two-dimensional position coordinate analysis. By shutting cells in a micro chamber, continuous microscopic observation of Navicula sp. cells was possible. The trajectory of each cell was visualized once every second by using position coordinate analysis although time resolution of previous papers were range of minutes. Our data revealed frequent change of movement direction. Furthermore, the correlation between the distances moved, the velocity, and the acceleration of the cells was discussed in detail.
我们通过结合微室和二维位置坐标分析来描述硅藻细胞运动的轨迹分析。通过将细胞困在微室中，可以连续观察 Navicula sp. 细胞。尽管以前的论文的时间分辨率在几分钟范围内，但通过位置坐标分析，我们可以每秒观察一次每个细胞的轨迹。我们的数据显示出运动方向的频繁变化。此外，还详细讨论了细胞移动的距离、速度和加速度之间的相关性。
