Wakelam Michael J O, Clark Jonathan
The Babraham Institute, Cambridge CB22 3AT, UK.
Biochim Biophys Acta. 2011 Nov;1811(11):758-62. doi: 10.1016/j.bbalip.2011.09.004. Epub 2011 Sep 23.
The polyphosphoinositides are key signaling lipids whose levels are tightly regulated within cells. As with other cellular lipids multiple species exist with distinct acyl chain makeups. There are methods which analyze the phosphoinositides as their deacylated derivatives which cannot address these distinct forms. Lipidomic analysis of the polyphosphoinositides has been hampered by difficulties with extraction and problems associated with binding of the lipids to surfaces. This review outlines the available MS methodologies, highlighting the difficulties associated with each. However, at present, no single methodology is available that can successfully and reproducibly quantitate each inositol phospholipid.
多磷酸肌醇是关键的信号脂质，其水平在细胞内受到严格调控。与其他细胞脂质一样，存在多种具有不同酰基链组成的种类。有一些方法将磷酸肌醇作为其脱酰基衍生物进行分析，但无法区分这些不同的形式。多磷酸肌醇的脂质组学分析因提取困难以及脂质与表面结合相关的问题而受到阻碍。本综述概述了可用的质谱方法，强调了每种方法所面临的困难。然而，目前尚无单一方法能够成功且可重复地定量每种肌醇磷脂。
