使用ω-炔基脂肪酸分析细胞肉豆蔻酰化和棕榈酰化。
Profiling cellular myristoylation and palmitoylation using ω-alkynyl fatty acids.
作者信息
Hannoush Rami N
机构信息
Department of Early Discovery Biochemistry, Genentech, Inc., South San Francisco, CA, USA.
出版信息
Methods Mol Biol. 2012;800:85-94. doi: 10.1007/978-1-61779-349-3_7.
PMID:21964784
Abstract
Methods to detect and characterize cellular protein myristoylation and palmitoylation are invaluable in cell biology, immunology, and virology. Recently, we developed ω-alkynyl fatty acid probes for monitoring myristoylation and palmitoylation of cellular proteins. This article describes a biochemical procedure for metabolic labeling of cells with ω-alkynyl fatty acids and click chemistry.
摘要
检测和表征细胞蛋白质肉豆蔻酰化和棕榈酰化的方法在细胞生物学、免疫学和病毒学中具有重要价值。最近，我们开发了ω-炔基脂肪酸探针来监测细胞蛋白质的肉豆蔻酰化和棕榈酰化。本文描述了一种用ω-炔基脂肪酸对细胞进行代谢标记和点击化学的生化程序。
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