Department of Pharmacotherapeutics, Faculty of Pharmacy, Keio University, 1-5-30 Sibakoen, Minato-Ku, Tokyo 105-0011, Japan.
Anticancer Res. 2011 Oct;31(10):3253-7.
Malignant glioma is an invasive disease of the central nervous system. One of the factors that regulate growth of these tumors is expression of epidermal growth factor receptor (EGFR) in the cells. This study investigated the effects of down-regulation of EGFR on cell proliferation, cell cycle and cytotoxicity to antineoplastic agent.
A short hairpin RNA transcription vector targeting EGFR was transfected into KNS42 cells. Growth curve, cell cycle and sensitivity to temozolomide of the cells were assessed.
Transfection inhibited EGFR expression by 50.5%. It prolonged cell doubling time by 25.7%. However, it did not meaningfully alter the cell cycle populations nor increase sensitivity to temozolomide.
Suppressing expression of EGFR inhibited cell proliferation. However, unlike PTEN expression or ROCK1 down-regulation, it did not alter the cell cycle or increase sensitivity to temozolomide.
恶性神经胶质瘤是一种中枢神经系统侵袭性疾病。调节这些肿瘤生长的因素之一是细胞中表皮生长因子受体（EGFR）的表达。本研究探讨了下调 EGFR 对细胞增殖、细胞周期和抗肿瘤药物细胞毒性的影响。
将靶向 EGFR 的短发夹 RNA 转录载体转染到 KNS42 细胞中。评估细胞的生长曲线、细胞周期和对替莫唑胺的敏感性。
转染抑制了 50.5%的 EGFR 表达。它将细胞倍增时间延长了 25.7%。然而，它并没有显著改变细胞周期群体，也没有增加对替莫唑胺的敏感性。
抑制 EGFR 的表达抑制了细胞增殖。然而，与 PTEN 表达或 ROCK1 下调不同，它并没有改变细胞周期或增加对替莫唑胺的敏感性。
