Department of Histopathology, Hammersmith Hospital Campus, Imperial College Healthcare NHS Trust, London, & Imperial College London, UK.
J Clin Pathol. 2013 Feb;66(2):155-9. doi: 10.1136/jclinpath-2011-200126. Epub 2011 Sep 30.
The authors aimed to develop a tool to assess total cell numbers in a microscope's field of vision, which would provide the denominator for calculating the percentage of positive cells for a given antigen in bone marrow trephine biopsies (BMTBs) of varying cellularities. Precise estimates of cell densities were made from 179 images of BMTBs of varying cellularities using a cell-counting software. The estimates were then validated on an independent set of 20 BMTBs. Among the 179 images, there was a strong linear association between marrow cellularity and cell numbers (Pearson correlation: 0.788). Then standardised cell densities (cells/mm(2) of bone marrow) were deduced for BMTBs of varying cellularities. In the validation study, the actual and the estimated cell numbers correlated strongly (Pearson correlation: 0.990). The cell density estimates provided in this study can be adapted for any microscope and the same method can be used for calculation of the percentage-positive cells for any antigen.
作者旨在开发一种工具来评估显微镜视野中的总细胞数，这将为计算骨髓穿刺活检（BMTB）中特定抗原的阳性细胞百分比提供分母，这些 BMTB 的细胞密度不同。使用细胞计数软件对 179 张不同细胞密度的 BMTB 图像进行了精确的细胞密度估计。然后，在一组 20 张独立的 BMTB 上对这些估计进行了验证。在 179 张图像中，骨髓细胞密度与细胞数量之间存在很强的线性关联（皮尔逊相关系数：0.788）。然后，针对不同细胞密度的 BMTB 推导出了标准化的细胞密度（骨髓中每毫米 2 的细胞数）。在验证研究中，实际和估计的细胞数量之间存在很强的相关性（皮尔逊相关系数：0.990）。本研究中提供的细胞密度估计值可适用于任何显微镜，并且可以使用相同的方法计算任何抗原的阳性细胞百分比。
