Department of Orthopaedics, Changzhou No. 1 People's Hospital, Changzhou, PR China.
Connect Tissue Res. 2012;53(2):142-8. doi: 10.3109/03008207.2011.614365. Epub 2011 Oct 3.
The purpose of this study was to compare the effects of spinal cord injury (SCI) and ovariectomy (OVX) on femoral fracture healing of later phase in young mice. Sixty young female C57 mice were randomized into three groups: SCI, OVX, and age-matched intact control. The femoral fracture was generated at 3 weeks after SCI or OVX. At 1 month after fracture, the femoral fracture area was evaluated through the healing status using radiograph; bone mineral density using dual X-ray absorptometry; callus formation and mineralization and neovascularization in callus using micro-computed tomography; biomechanical analysis using testing machine; and histology analysis by staining with hematoxylin-eosin stain. SCI mice showed lower bone mineral density in the femoral callus as compared with OVX mice. Callus geometric microstructural parameters of the femora in SCI mice were significantly lower than OVX mice. SCI induced significant changes of biomechanical parameters in the femoral fracture healing area. The callus formation and callus neovascularization in SCI mice were significantly lower than in OVX mice. SCI induces more deterioration of fracture healing in the femoral diaphysis than OVX.
本研究旨在比较脊髓损伤（SCI）和卵巢切除（OVX）对年轻小鼠后期股骨骨折愈合的影响。60 只年轻雌性 C57 小鼠随机分为三组：SCI、OVX 和年龄匹配的完整对照组。SCI 或 OVX 后 3 周生成股骨骨折。骨折后 1 个月，通过 X 线评估骨折愈合状态；双能 X 线吸收法评估骨密度；微计算机断层扫描评估骨痂形成和矿化及骨痂内新生血管；试验机进行生物力学分析；以及通过苏木精-伊红染色进行组织学分析。与 OVX 小鼠相比，SCI 小鼠的股骨骨痂骨密度较低。SCI 小鼠的股骨骨痂几何微观结构参数明显低于 OVX 小鼠。SCI 导致股骨骨折愈合区域的生物力学参数发生显著变化。SCI 小鼠的骨痂形成和骨痂新生血管明显低于 OVX 小鼠。SCI 导致股骨骨干骨折愈合的恶化程度高于 OVX。
