Department of Otorhinolaryngology Head and Neck Surgery, Yantai Yu-huang-ding Hospital, No. 20, Yu-huang-ding Dong Road, Yantai, 264000, People's Republic of China.
Eur Arch Otorhinolaryngol. 2012 Feb;269(2):557-63. doi: 10.1007/s00405-011-1786-5. Epub 2011 Oct 4.
To better evaluate the serum protein alterations in patients with laryngeal carcinoma during surgical treatment process, a comparative proteomic analysis of human serum from patients with laryngeal carcinoma between pre- and post-operation group was performed using two-dimensional gel electrophoresis, in combination with matrix-assisted laser desorption ionization time-of-flight mass spectrometry and database searching. Statistical analysis indicated that 16 gel spots corresponding to 12 proteins altered their expression significantly between the two groups of patients with laryngeal carcinoma. Of these 12 proteins, 6 proteins were up-regulated in the pre-operation group. The subsequent Western blot confirmed the results of proteome analysis. Measurement of MDA and SOD levels in serum combining with bioinformatics analysis indicated the potential roles of the oxidant stress and immune response as target in monitoring and treating laryngeal carcinoma. The study provides new insight into the laryngeal carcinoma development and treatment, and the identified proteins are warranted to be further studied.
为了更好地评估喉癌患者在手术治疗过程中的血清蛋白变化，本研究采用二维凝胶电泳结合基质辅助激光解吸电离飞行时间质谱和数据库搜索的方法，对术前和术后两组喉癌患者的血清进行了比较蛋白质组学分析。统计分析表明，两组喉癌患者的血清中有 16 个凝胶斑点所对应的 12 种蛋白质的表达有显著差异。这 12 种蛋白质中，有 6 种在术前组中上调。随后的 Western blot 验证了蛋白质组分析的结果。结合生物信息学分析测量血清中的 MDA 和 SOD 水平，表明氧化应激和免疫反应可能作为监测和治疗喉癌的靶点。该研究为喉癌的发展和治疗提供了新的视角，所鉴定的蛋白质值得进一步研究。
