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酵母3-磷酸甘油酸激酶启动子在大肠杆菌中转录活性的温度依赖性

Temperature dependence of transcriptional activity of yeast 3-phosphoglycerokinase promoter in Escherichia coli.

作者信息

Mondal A K, Roy T K

机构信息

Institute of Microbial Technology, Chandigarh, India.

出版信息

Biochem Biophys Res Commun. 1990 Jul 16;170(1):1-9. doi: 10.1016/0006-291x(90)91232-h.

DOI:10.1016/0006-291x(90)91232-h
PMID:2196875
Abstract

We have studied the activity of yeast PGK promoter in E. coli at different temperatures using lacZ as the reporter gene. The B-galactosidase activity was found to be less at 42 degrees C than at 30 degrees C. Northern blot analysis showed that the level of lacZ transcript was less in recombinant cells grown at 42 degrees C, whereas plasmid copy number per cell was more as compared to recombinant cells grown at 30 degrees C. Data suggest that the yeast PGK promoter is less active at 42 degrees C and that this activity is regulated at the level of transcription.

摘要

我们使用lacZ作为报告基因，研究了酵母PGK启动子在不同温度下在大肠杆菌中的活性。发现β-半乳糖苷酶活性在42℃时低于30℃时。Northern印迹分析表明，在42℃生长的重组细胞中lacZ转录本水平较低，而与在30℃生长的重组细胞相比，每个细胞的质粒拷贝数更多。数据表明酵母PGK启动子在42℃时活性较低，且这种活性在转录水平受到调控。

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