Zhou Guangjin, Liu Yifei, Wu Shwu-Yuan, Tie Feng, Lou Hua, Chiang Cheng-Ming, Luo Guangbin
Int J Biochem Mol Biol. 2010;1(1):101-111. Epub 2010 Jul 15.
RecQ helicases are members of an evolutionary conserved family of DNA helicases. They are homologous to the RecQ helicase of E. coli, the founding member of the family. These enzymes include gene products of disease-causing genes in Bloom, Werner, and Rothmund-Thomson syndrome. To date, these proteins have been implicated in many aspects of DNA metabolism, including DNA replication, repair, and recombination. We reported here that RECQL5, a newer member of the human RecQ helicase family, physically interacts with SWI/SNF complex and RNAPII core complex within the context of a super complex. RECQL5 was detected in the RNAPII holoenzyme but not in purified RNAPII core complex. Together, these data link RECQL5 to the assembly of the RNAPII transcription machinery and suggest that this helicase may have a regulatory role in RNAPII transcription or an RNAPII-related process or processes.
RecQ解旋酶是DNA解旋酶进化保守家族的成员。它们与该家族的创始成员——大肠杆菌的RecQ解旋酶同源。这些酶包括布卢姆综合征、沃纳综合征和罗思蒙德-汤姆森综合征中致病基因的基因产物。迄今为止，这些蛋白质已涉及DNA代谢的许多方面，包括DNA复制、修复和重组。我们在此报告，人类RecQ解旋酶家族的新成员RECQL5在一个超级复合物的背景下与SWI/SNF复合物和RNAPII核心复合物发生物理相互作用。在RNAPII全酶中检测到了RECQL5，但在纯化的RNAPII核心复合物中未检测到。这些数据共同将RECQL5与RNAPII转录机制的组装联系起来，并表明这种解旋酶可能在RNAPII转录或一个或多个与RNAPII相关的过程中发挥调节作用。
