Division of Toxicology and Risk Assessment, National Food Institute, Technical University of Denmark, Mørkhøj Bygade 19, 2860 Søborg, Denmark.
Arch Toxicol. 2012 Apr;86(4):543-51. doi: 10.1007/s00204-011-0759-1. Epub 2011 Oct 4.
Subacute toxicity of 14 nm nanoparticulate silver (Ag-NP) stabilised with polyvinylpyrrolidone and ionic silver in the form of silver acetate (Ag-acetate) was investigated in four-week-old Wistar rats. Animals received orally by gavage the following: vehicle control (10 ♀, 6 ♂); Ag-NP at doses: 2.25 (8 ♀), 4.5 (8 ♀) or 9 mg/kg bw/day (10 ♀, 6 ♂); or Ag-acetate 9 mg silver/kg bw/day (8 ♀) for 28 days. Clinical, haematolological and biochemical parameters, organ weights, macro- and microscopic pathological changes were investigated. Caecal bacterial phyla and their silver resistance genes were quantified. For the Ag-NP groups, no toxicological effects were recorded. For Ag-acetate, lower body weight gain (day 4-7, 11-14, 14-16, P < 0.05; overall, day 1-28, P < 0.01), increased plasma alkaline phosphatase (P < 0.05), decreased plasma urea (P < 0.05) and lower absolute (P < 0.01) and relative (P < 0.05) thymus weight were recorded. In conclusion, these findings indicate toxicity of 9 mg/kg bw/day ionic silver but not of an equimolar Ag-NP dose. This is in accordance with previously reported data showing that oral Ag-acetate, in comparison with an equimolar dose of Ag-NP, resulted in higher silver plasma and organ concentrations.
研究了聚维酮稳定的 14nm 纳米银颗粒（Ag-NP）和醋酸银（Ag-acetate）形式的离子银在四周龄 Wistar 大鼠中的亚急性毒性。动物通过灌胃接受以下处理：载体对照（10 只♀，6 只♂）；Ag-NP 剂量分别为：2.25（8 只♀）、4.5（8 只♀）或 9mg/kg bw/day（10 只♀，6 只♂）；或 9mg/kg bw/day 的 Ag-acetate（8 只♀），共 28 天。检测了临床、血液学和生物化学参数、器官重量、宏观和微观病理变化。还定量了盲肠细菌门及其银抗性基因。对于 Ag-NP 组，未记录到毒性作用。对于 Ag-acetate，体重增加减少（第 4-7 天、第 11-14 天、第 14-16 天，P<0.05；整体上第 1-28 天，P<0.01），血浆碱性磷酸酶升高（P<0.05），血浆尿素降低（P<0.05），胸腺绝对重量降低（P<0.01），相对重量降低（P<0.05）。总之，这些发现表明 9mg/kg bw/day 离子银具有毒性，但等摩尔剂量的 Ag-NP 没有毒性。这与之前的报道数据一致，表明与等摩尔剂量的 Ag-NP 相比，口服 Ag-acetate 会导致更高的银血浆和器官浓度。
