Bonatto Cínthia Caetano, Reis Ivy Garcez, Ribeiro Dalila Juliana Silva, Almeida Raquel das Neves, Corrêa Rafael, Dourado Livia Pimentel Sant'Ana, Pasquarelli-do-Nascimento Gabriel, Magalhães Kelly Grace, Silva Luciano Paulino
Postgraduate Program in Animal Biology, University of Brasilia (UnB), Brasília 70910-900, DF, Brazil.
Laboratory of Nanobiotechnology (LNANO), Embrapa Genetic Resources and Biotechnology, Parque Estacao Biologica, Final W5 Norte, Brasília 70770-917, DF, Brazil.
Pharmaceuticals (Basel). 2025 Jul 2;18(7):993. doi: 10.3390/ph18070993.
In recent years, silver nanoparticles (AgNPs) have garnered significant attention due to their potent antimicrobial properties, which hold promise for various applications. However, concerns about their potential toxicity have also emerged, particularly regarding their impact on human and animal health. This study investigates the acute toxicological effects of AgNPs synthesized using a green route with an aqueous extract of a native Cerrado plant (AgNPs-Cb) in mice. : The AgNPs-Cb were intravenously administered at a concentration of 64 µM, and the mice were euthanized after 24 h for the collection of blood and organ samples (liver, spleen, kidneys, and lungs) for hematological, biochemical, and histological analyses. Hematological analysis, including complete blood count (CBC) and differential leukocyte count, showed no statistically significant alterations in the groups treated with AgNPs-Cb, Cb extract, and Ag, compared with the control group ( < 0.05). Notably, only the Ag group exhibited a significant increase in red blood cell count and hematocrit levels, suggesting that the nanoformulation of silver might mitigate the hematological impact seen with free silver ions. Biochemical analyses of liver and kidney function markers also revealed no significant differences across the treatment groups. These findings indicate that AgNPs-Cb may offer a safer alternative for antimicrobial applications, reducing the risk of acute toxicity in mammals while maintaining efficacy against pathogens. Further studies are needed to explore the underlying mechanisms and long-term effects of AgNPs-Cb exposure.
近年来，银纳米颗粒（AgNPs）因其强大的抗菌性能而备受关注，这些性能在各种应用中都具有潜力。然而，人们也开始关注其潜在毒性，特别是对人类和动物健康的影响。本研究调查了使用巴西塞拉多地区一种本土植物的水提取物通过绿色途径合成的银纳米颗粒（AgNPs-Cb）对小鼠的急性毒理学效应。将浓度为64µM的AgNPs-Cb静脉注射给小鼠，24小时后对小鼠实施安乐死，以采集血液和器官样本（肝脏、脾脏、肾脏和肺）用于血液学、生化和组织学分析。血液学分析，包括全血细胞计数（CBC）和白细胞分类计数，结果显示，与对照组相比，用AgNPs-Cb、Cb提取物和银处理的组没有统计学上的显著变化（<0.05）。值得注意的是，只有银组的红细胞计数和血细胞比容水平显著增加，这表明银的纳米制剂可能减轻游离银离子对血液学的影响。肝脏和肾脏功能标志物的生化分析也显示各治疗组之间没有显著差异。这些发现表明，AgNPs-Cb可能为抗菌应用提供一种更安全的选择，在维持对病原体有效性的同时降低哺乳动物急性毒性的风险。需要进一步研究以探索AgNPs-Cb暴露的潜在机制和长期影响。
