Gomazkov O A
Nauchnye Doki Vyss Shkoly Biol Nauki. 1990(3):5-20.
An attempt to treat critically the key methodological positions of modern biochemistry, primarily, the medical biochemistry with reference to the physiologic, pathophysiologic and clinical tasks is made. The author puts forward questions: 1) if it is possible to apply traditional approaches (principles) of biochemical investigation developed on purified systems to the whole organism systems in situ and etc.; 2) if it coincides the methodical approaches of biochemistry on the one hand and physiology (pathophysiology)--on the other one, considering the mechanisms of disease development and correspondingly the assessment and choice of therapeutic methods; 3) if there exists a necessity to create a unite methodology of biochemical and physiologic knowledge as a basis of modern medical science as a whole. Some examples showing the possible ways of new methodic consideration of traditional conceptions of in vitro biochemistry are given.
本文尝试批判性地探讨现代生物化学的关键方法论立场，主要是涉及生理、病理生理及临床任务的医学生物化学。作者提出了以下问题：1）是否有可能将在纯化系统中发展起来的传统生化研究方法（原则）应用于原位的整个生物体系统等；2）考虑到疾病发展机制以及相应的治疗方法评估和选择，生物化学的方法论方法与生理学（病理生理学）的方法论方法是否一致；3）是否有必要创建一种统一的生化和生理知识方法论，作为整个现代医学科学的基础。文中给出了一些示例，展示了对体外生物化学传统概念进行新方法思考的可能途径。
