Department of Physiology and Functional Genomics, University of Florida, Gainesville, FL 32610, USA.
J Physiol. 2011 Dec 15;589(Pt 24):6129-38. doi: 10.1113/jphysiol.2011.214569. Epub 2011 Oct 3.
The ageing kidney exhibits slowly developing chronic kidney disease (CKD) and is associated with nitric oxide (NO) deficiency and increased oxidative stress. The impact of exercise on the ageing kidney is not well understood. Here, we determined whether 12 weeks of treadmill exercise can influence age-dependent CKD in old (22-24 months) Fisher 344 (F344) male rats by comparing sedentary (SED) and exercise (EX) trained rats; young (3 months) rats were also studied. In addition to renal structure and function, we assessed protein levels of various isoforms of the NO synthases (NOS) and superoxide dismutase (SOD) enzymes as well as markers of oxidative stress, in kidney cortex and medulla. Renal function as determined by plasma creatinine, proteinuria, and glomerular structural injury worsened with age and was unaffected by exercise. Ageing also increased the protein abundance of neuronal NOSβ and p22phox while decreasing extracellular (EC) and copper/zinc (CuZn) SOD, in kidney cortex and medulla. H(2)O(2) content and nitrotyrosine abundance also increased in the kidney with age. None of these age-related changes were altered with exercise. However, exercise did increase renal cortical endothelial (e)NOS and EC SOD in young rats. Data indicate that exercise-induced increases in eNOS and EC SOD seen in young rats are lost with age. We conclude that chronic exercise is ineffective in reversing age-dependent CKD in the male F344 rat.
衰老的肾脏表现出缓慢发展的慢性肾脏病 (CKD)，并伴有一氧化氮 (NO) 缺乏和氧化应激增加。运动对衰老肾脏的影响尚未得到充分了解。在这里，我们通过比较久坐不动 (SED) 和运动 (EX) 训练的大鼠，确定 12 周的跑步机运动是否可以影响老年 (22-24 个月) Fisher 344 (F344) 雄性大鼠的年龄依赖性 CKD；还研究了年轻 (3 个月) 大鼠。除了肾脏结构和功能外，我们还评估了肾脏皮质和髓质中各种 NO 合酶 (NOS) 和超氧化物歧化酶 (SOD) 同工型的蛋白水平以及氧化应激标志物。肾功能由血浆肌酐、蛋白尿和肾小球结构损伤确定，随年龄增长而恶化，不受运动影响。衰老还增加了神经元 NOSβ 和 p22phox 的蛋白丰度，同时降低了肾脏皮质和髓质中的细胞外 (EC) 和铜/锌 (CuZn) SOD。随着年龄的增长，肾脏中的 H(2)O(2)含量和硝基酪氨酸含量也增加。这些与年龄相关的变化都没有因运动而改变。然而，运动确实增加了年轻大鼠肾脏皮质的内皮 (e)NOS 和 EC SOD。数据表明，年轻大鼠中运动诱导的 eNOS 和 EC SOD 增加随着年龄的增长而丧失。我们得出结论，慢性运动不能逆转雄性 F344 大鼠的年龄依赖性 CKD。
