Chen Jing-Jie, Li Yong, He Han, Su Ling-Song, Qin Song-Shu, Hu Hong-Bo, Tang Liu-Sheng, Li Shi-Li
Department of Clinical Laboratory, Longtan Hospital, Liuzhou 545005, China.
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 2011 Jun;29(3):176-8.
Pneumocystis jirovecii was detected in sputum samples and bronchoalveolar lavage fluid (BALF) obtained from HIV/AIDS patients complicated with Pneumocystis jirovecii pneumonia by Giemsa staining. CD4+ T lymphocytes of 500 patients were counted by flow cytometer. P. jirovecii positive rate in sputum samples (46.8%, 845/1 806) significantly lower than that of BALF (55.8%, 10(6)/190) (P < 0.05). The proportion of patients developing clinical symptoms in P. jirovecii positive cases (96.6%, 816/845) was higher than that of P. jirovecii negative cases (64.0%, 615/961) (P < 0.05). P. jirovecii positive rate increased with the decrease of CD4+ T lymphocyte number. P. jirovecii positive rates in cases with CD4+ > 200 x 10(6)/L, CDC 200 x 10(6)/L-100 x 10(6)n/L, and CD4+ < 100x10(6)/12.0% (6/50), 39.0%( 39/100), 54.6% (191/350), respectively (P < 0.05). Giemsa staining is an efficient, simple and feasible method for P. jirovecii detection, relying on the experience and skill of the operator.
通过吉姆萨染色法在合并耶氏肺孢子菌肺炎的艾滋病患者的痰液样本和支气管肺泡灌洗（BALF）液中检测到耶氏肺孢子菌。采用流式细胞仪对500例患者的CD4+ T淋巴细胞进行计数。痰液样本中耶氏肺孢子菌阳性率为46.8%（845/1806），显著低于BALF中的阳性率55.8%（1006/190）（P<0.05）。耶氏肺孢子菌阳性病例中出现临床症状的患者比例（96.6%，816/845）高于耶氏肺孢子菌阴性病例（64.0%，615/961）（P<0.05）。耶氏肺孢子菌阳性率随CD4+ T淋巴细胞数量的减少而升高。CD4+>200×10⁶/L、200×10⁶/L - 100×10⁶/L、CD4+<100×10⁶/L病例中的耶氏肺孢子菌阳性率分别为12.0%（6/50）、39.0%（39/100）、54.6%（191/350）（P<0.05）。吉姆萨染色法是一种检测耶氏肺孢子菌的有效、简单且可行的方法，但依赖于操作人员的经验和技能。
