Bao Yong-Xing, Mao Rui, Qi Hong-Zhi, Zhang Yue-Fen, Ni Ya-Qiong, Xie Zeng-Ru, Aziguli Tursun, Wen Hao
Cancer Center of the First Teaching Hospital, Xinjiang Medical University, Urumqi 830054, China.
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 2011 Jun;29(3):208-11.
To explore the effect of X-ray irradiation on Echinococcus multilocularis protoscoleces in vitro.
Echinococcus multilocularis protoscoleces were collected from cysts of infected Meriones meridianus and then cultured in RPMI 1640 medium. Protoscoleces were subpackaged into culture flasks at a density of about 10(4) per flask after culture for 3 days. Each group has 10 culture flasks. There were seven groups named as blank control group, low dose group (15 Gy and 30 Gy), medium dose group (45 Gy and 60 Gy), high dose group (75 Gy and 90 Gy), albendazole group (2 500 ng/ml), 45 Gy X-ray + 2 500 ng/ml albendazole group, and 75 Gy X-ray + 2 500 ng/ml albendazole group. Protoscoleces received three radiations on every other day with a source-skin distance of 100 cm and at a dose rate of 200 cGy/min after 3 days in culture. At each day after irradiation, protoscoleces were counted by light microscope with 0.1% eosin staining, and calculated mortality rate (per 100 protoscoleces) until all the parasites in experimental groups died. At the same time, the morphological changes of protoscoleces were observed.
There were significant differences in protoscolex mortality between X-ray groups and blank control group (P < 0.05), between X-ray + albendazole groups and albendazole group (P < 0.05). Protoscolex mortality in albendazole group were higher than that of blank control group (P < 0.05). Significant difference were also found in protoscolex mortality between albendazole combined with radiation and radiation only (P < 0.05). Before radiation, protoscoleces was normal with complete structure. After radiation, the parasites were mostly valgus type protoscoleces with disordered rostellar hooks and deformed acetabulum, and finally died.
X-ray can kill Echinococcus multilocularis protoscoleces in vitro.
探讨X射线体外照射对多房棘球绦虫原头节的影响。
从感染子午沙鼠的囊肿中收集多房棘球绦虫原头节，然后在RPMI 1640培养基中培养。培养3天后，将原头节以每瓶约10⁴的密度分装到培养瓶中。每组有10个培养瓶。分为7组，分别为空白对照组、低剂量组（15 Gy和30 Gy）、中剂量组（45 Gy和60 Gy）、高剂量组（75 Gy和90 Gy）、阿苯达唑组（2500 ng/ml）、45 Gy X射线 + 2500 ng/ml阿苯达唑组、75 Gy X射线 + 2500 ng/ml阿苯达唑组。培养3天后，原头节每隔一天接受3次照射，源皮距离为100 cm，剂量率为200 cGy/min。照射后每天用0.1%伊红染色，在光学显微镜下计数原头节，并计算死亡率（每100个原头节），直至实验组所有寄生虫死亡。同时，观察原头节的形态变化。
X射线组与空白对照组之间原头节死亡率有显著差异（P < 0.05），X射线 + 阿苯达唑组与阿苯达唑组之间有显著差异（P < 0.05）。阿苯达唑组原头节死亡率高于空白对照组（P < 0.05）。阿苯达唑联合照射组与单纯照射组原头节死亡率也有显著差异（P < 0.05）。照射前，原头节结构完整，形态正常。照射后，寄生虫大多为外翻型原头节，顶突钩排列紊乱，吸盘变形，最终死亡。
X射线可在体外杀死多房棘球绦虫原头节。
