Tielemans Y, Willems G, Sundler F, Håkanson R
Cancer Research Unit, Faculty of Medicine, Vrije Universiteit Brussels, Belgium.
Digestion. 1990;45(3):138-46. doi: 10.1159/000200235.
The renewal mechanisms for enterochromaffin-like (ECL) cells, the predominating endocrine cell population in the oxyntic mucosa of the stomach, were investigated in the mouse. The ECL cells were selectively demonstrated by immunostaining using histamine antibodies. Under basal conditions, when observed during the night, ECL cells in mitosis could be seen. This observation proved their ability to divide. Autoradiography after a single pulse and after multiple injections of 3H-thymidine made it possible to study some of their cytokinetic characteristics. The observed replication rate of the labeled ECL cells suggested that self-replication is the main mechanism by which the ECL cell population is renewed. The time interval between two successive divisions of labeled ECL cells was estimated to be around 60 days. Since ECL cells proliferate through mitosis, it may be expected that specific mitogenic stimuli might promote the induction of ECL cell hyperplasia and eventually ECL cell tumors (gastric carcinoids).
在小鼠中研究了胃泌酸黏膜中占主导地位的内分泌细胞群——肠嗜铬样（ECL）细胞的更新机制。使用组胺抗体通过免疫染色选择性地显示ECL细胞。在基础条件下，在夜间观察时，可以看到处于有丝分裂期的ECL细胞。这一观察结果证明了它们的分裂能力。单次脉冲注射和多次注射3H-胸腺嘧啶后的放射自显影使得研究它们的一些细胞动力学特征成为可能。观察到的标记ECL细胞的复制率表明自我复制是ECL细胞群更新的主要机制。标记的ECL细胞连续两次分裂之间的时间间隔估计约为60天。由于ECL细胞通过有丝分裂增殖，可以预期特定的促有丝分裂刺激可能会促进ECL细胞增生的诱导，并最终导致ECL细胞瘤（胃类癌）。
