免疫荧光抗体试验、酶免疫测定法及免疫印迹法检测HIV-1抗体的比较评估

Comparative evaluation of an immunofluorescent antibody test, enzyme immunoassay and western blot for the detection of HIV-1 antibody.

作者信息

Iltis J P, Patel N M, Lee S R, Barmat S L, Wallen W C

机构信息

Wampole Laboratories and Carter-Wallace Diagnostics, Cranbury, N.J. 08512.

出版信息

Intervirology. 1990;31(2-4):122-8. doi: 10.1159/000150146.

DOI:10.1159/000150146

PMID:2197249

Abstract

Screening blood and blood products for human immunodeficiency virus type 1 (HIV-1) antibody is predominantly performed by enzyme immunoassay (EIA), and results must be confirmed by the more immunospecific Western blot (WB) assay. This study evaluated an HIV immunofluorescent antibody (IFA) test relative to WB assay for use in confirming EIA designated HIV-1 antibody-positive sera. Specimens from seroconversion and CDC panels as well as clinical specimens obtained for routine EIA HIV-1 antibody screening were evaluated. Results with 209 specimens indicated that sensitivity and specificity of the Fluorognost-HIV assay were equivalent relative to WB. In addition, the Fluorognost-HIV IFA test was faster and easier to perform than the WB assay, and unlike the WB assay was not prone to indeterminate results.

摘要

对人类免疫缺陷病毒1型（HIV-1）抗体进行血液和血液制品筛查主要采用酶免疫测定（EIA）法，其结果必须通过特异性更强的免疫印迹法（WB）进行确认。本研究评估了一种HIV免疫荧光抗体（IFA）检测法相对于WB法在确认EIA检测指定为HIV-1抗体阳性血清中的应用。对血清转化和疾病控制中心检测组的标本以及为常规EIA HIV-1抗体筛查而获取的临床标本进行了评估。209份标本的检测结果表明，Fluorognost-HIV检测法相对于WB法的敏感性和特异性相当。此外，Fluorognost-HIV IFA检测法比WB法更快且操作更简便，而且与WB法不同的是，它不易出现不确定结果。