[L-精氨酸通过激活树突状细胞增强DBA/2小鼠对约氏疟原虫17XL感染的Th1免疫反应]
[L-arginine enhances Th1 immune response against Plasmodium yoelii 17XL infection in DBA/2 mice via activation of dendritic cells].
作者信息
Pan Yan-yan, Liu Jun, Li Ying, Yan Juan, Cao Ya-ming
机构信息
Department of Immunology, College of Basic Medical Sciences, China Medical University, Shenyang, 110001 China.
出版信息
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 2011 Aug;29(4):247-51.
OBJECTIVE
To investigate the effect of L-arginine (L-Arg) during blood-stage infection by P.y17XL in DBA/2 mice.
METHODS
DBA/2 mice were divided into 2 groups, 20 mice in each group. Mice were respectively administered with L-Arg (1.5 g/kg, L-Arg group) and normal saline (control group) 7 days before they were infected intraperitoneally by 1 x 10(6) pRBC. Parasitemia were detected by Giemsa stained thin-smear microscopy and survival rate were monitored daily. Flow cytometry was introduced to detect the subsets of splenic CD4+CD69+ T cells, F4/80+CD36+ macrophages, myeloid dendritic cells (mDCs) (CD11c+CD11b+), plasmacytoid dendritic cells (pDCs) (CD11c+B220+) on day 3, 5 post infection (p.i.). The levels of IFN-gamma and NO in the supernatant of splenocytes culture were detected by ELISA and Griess reaction, respectively.
RESULTS
Pre-treatment of mice with L-Arg significantly decreased the parasitemia from 45% to 20% and shortened self-cure time from 22d to 20d after infection. The level of F4/80+CD36+ macrophages [(29.61 +/- 0.47)%], IFN-gamma [(485.84 +/- 39.31) pg/ml], CD4+CD69+ T cells [(7.3 +/- 0.68)%], NO [(42.51 +/- 1.32) micromol/L], mDCs(CD11c+CD11b+) [(5.51 +/- 0.87)%] and pDCs(CD11c+B220+) [(5.60 +/- 0.85)%] in L-Arg group was higher than those in control group [(36.46 +/- 1.33)%, (767.86 +/- 20.56) pg/ml, (11.27 +/- 0.97)%, (78.66 +/- 2.89) micromol/L, (10.02 +/- 0.37)%] and (9.01 +/- 0.53)%, respectively].
CONCLUSION
L-Arg enhances Th1 immune responses during the early stage of P.y17XL infection in DBA/2 mice via the activation of DCs.
目的
研究L-精氨酸（L-Arg）对DBA/2小鼠血液期约氏疟原虫（P.y17XL）感染的影响。
方法
将DBA/2小鼠分为2组，每组20只。在小鼠腹腔注射1×10(6)个感染疟原虫的红细胞前7天，分别给予L-Arg（1.5 g/kg，L-Arg组）和生理盐水（对照组）。通过吉姆萨染色薄涂片显微镜检测疟原虫血症，并每日监测存活率。在感染后第3天、第5天，采用流式细胞术检测脾脏CD4+CD69+ T细胞、F4/80+CD36+巨噬细胞、髓样树突状细胞（mDCs）（CD11c+CD11b+）、浆细胞样树突状细胞（pDCs）（CD11c+B220+）亚群。分别采用ELISA和Griess反应检测脾细胞培养上清中IFN-γ和NO水平。
结果
L-Arg预处理小鼠可使感染后疟原虫血症从45%显著降低至20%，自愈时间从22天缩短至20天。L-Arg组F4/80+CD36+巨噬细胞水平[（29.61±0.47）%]、IFN-γ水平[（485.84±39.31）pg/ml]、CD4+CD69+ T细胞水平[（7.3±0.68）%]、NO水平[（42.51±1.32）μmol/L]、mDCs（CD11c+CD11b+）水平[（5.51±0.87）%]和pDCs（CD11c+B220+）水平[（5.60±0.85）%]均高于对照组[分别为（36.46±1.33）%、（767.86±20.56）pg/ml、（11.27±0.97）%、（78.66±2.89）μmol/L、（10.02±0.37）%和（9.01±0.53）%]。
结论
L-Arg通过激活树突状细胞增强DBA/2小鼠P.y17XL感染早期的Th1免疫反应。
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