Pan Yan-yan, Liu Jun, Li Ying, Yan Juan, Cao Ya-ming
Department of Immunology, College of Basic Medical Sciences, China Medical University, Shenyang, 110001 China.
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 2011 Aug;29(4):247-51.
To investigate the effect of L-arginine (L-Arg) during blood-stage infection by P.y17XL in DBA/2 mice.
DBA/2 mice were divided into 2 groups, 20 mice in each group. Mice were respectively administered with L-Arg (1.5 g/kg, L-Arg group) and normal saline (control group) 7 days before they were infected intraperitoneally by 1 x 10(6) pRBC. Parasitemia were detected by Giemsa stained thin-smear microscopy and survival rate were monitored daily. Flow cytometry was introduced to detect the subsets of splenic CD4+CD69+ T cells, F4/80+CD36+ macrophages, myeloid dendritic cells (mDCs) (CD11c+CD11b+), plasmacytoid dendritic cells (pDCs) (CD11c+B220+) on day 3, 5 post infection (p.i.). The levels of IFN-gamma and NO in the supernatant of splenocytes culture were detected by ELISA and Griess reaction, respectively.
Pre-treatment of mice with L-Arg significantly decreased the parasitemia from 45% to 20% and shortened self-cure time from 22d to 20d after infection. The level of F4/80+CD36+ macrophages [(29.61 +/- 0.47)%], IFN-gamma [(485.84 +/- 39.31) pg/ml], CD4+CD69+ T cells [(7.3 +/- 0.68)%], NO [(42.51 +/- 1.32) micromol/L], mDCs(CD11c+CD11b+) [(5.51 +/- 0.87)%] and pDCs(CD11c+B220+) [(5.60 +/- 0.85)%] in L-Arg group was higher than those in control group [(36.46 +/- 1.33)%, (767.86 +/- 20.56) pg/ml, (11.27 +/- 0.97)%, (78.66 +/- 2.89) micromol/L, (10.02 +/- 0.37)%] and (9.01 +/- 0.53)%, respectively].
L-Arg enhances Th1 immune responses during the early stage of P.y17XL infection in DBA/2 mice via the activation of DCs.
研究L-精氨酸（L-Arg）对DBA/2小鼠血液期约氏疟原虫（P.y17XL）感染的影响。
将DBA/2小鼠分为2组，每组20只。在小鼠腹腔注射1×10(6)个感染疟原虫的红细胞前7天，分别给予L-Arg（1.5 g/kg，L-Arg组）和生理盐水（对照组）。通过吉姆萨染色薄涂片显微镜检测疟原虫血症，并每日监测存活率。在感染后第3天、第5天，采用流式细胞术检测脾脏CD4+CD69+ T细胞、F4/80+CD36+巨噬细胞、髓样树突状细胞（mDCs）（CD11c+CD11b+）、浆细胞样树突状细胞（pDCs）（CD11c+B220+）亚群。分别采用ELISA和Griess反应检测脾细胞培养上清中IFN-γ和NO水平。
L-Arg预处理小鼠可使感染后疟原虫血症从45%显著降低至20%，自愈时间从22天缩短至20天。L-Arg组F4/80+CD36+巨噬细胞水平[（29.61±0.47）%]、IFN-γ水平[（485.84±39.31）pg/ml]、CD4+CD69+ T细胞水平[（7.3±0.68）%]、NO水平[（42.51±1.32）μmol/L]、mDCs（CD11c+CD11b+）水平[（5.51±0.87）%]和pDCs（CD11c+B220+）水平[（5.60±0.85）%]均高于对照组[分别为（36.46±1.33）%、（767.86±20.56）pg/ml、（11.27±0.97）%、（78.66±2.89）μmol/L、（10.02±0.37）%和（9.01±0.53）%]。
L-Arg通过激活树突状细胞增强DBA/2小鼠P.y17XL感染早期的Th1免疫反应。
