Department of Chemistry, University of British Columbia, Vancouver, BC, Canada.
Anal Chem. 2011 Nov 15;83(22):8748-55. doi: 10.1021/ac202130f. Epub 2011 Oct 24.
A capillary electrophoresis mass spectrometry (CE-MS) interface utilizing a flow-through microvial is used to ensure the electric continuity and supply the catholyte and mobilizer solutions during the capillary isoelectric focusing (cIEF) and mobilization process. The flow-through microvial provides a stable chemical environment and helps to improve the ionization efficiency without significantly diluting the analyte. The CE-MS interface facilitates the transfer of the mobilized cIEF effluent to the site of electrospray ionization, and the gaseous ions can be detected directly by a mass spectrometer. It also allows for complete focusing and mobilization processes to be performed automatically in programmed sequences with commercial CE systems. Two different strategies, using either a part of the capillary or the flow-through microvial of the CE-MS interface as the catholyte reservoir for bare fused silica capillaries or neutral coated capillaries, respectively, were developed for automated cIEF-electrospray ionization (ESI)-MS. Reasonable separation efficiency was achieved using proper concentration of carrier ampholytes and suitable strategies of electroosmotic/electrophoretic mobilization.
采用流通微管的毛细管电泳-质谱（CE-MS）接口可确保在毛细管等电聚焦（cIEF）和迁移过程中保持电连续性并提供阴极液和迁移液。流通微管提供了稳定的化学环境，有助于提高离子化效率，而不会显著稀释分析物。CE-MS 接口可促进将迁移的 cIEF 流出物转移到电喷雾电离的位置，并且气态离子可以直接通过质谱仪进行检测。它还允许在商业 CE 系统中以编程序列自动执行完整的聚焦和迁移过程。分别使用 CE-MS 接口的一部分毛细管或流通微管作为裸熔融石英毛细管或中性涂层毛细管的阴极液储液器，开发了两种不同的策略，用于自动化 cIEF-电喷雾电离（ESI）-MS。通过使用适当浓度的载体两性电解质和合适的电渗流/电泳迁移策略，实现了合理的分离效率。
