Buesgen-Institute, Department of Forest Genetics and Forest Tree Breeding, University of Göttingen, Germany.
Plant Biol (Stuttg). 2011 Nov;13(6):881-7. doi: 10.1111/j.1438-8677.2011.00446.x. Epub 2011 Feb 11.
For the first time in sessile oak [Quercus petraea (Matt.) Liebl.], the isolation and characterisation of a full-length dehydrin gene and its promoter region, as well as its allelic variation in natural populations, is reported. Dehydrins (Dhn) are stress-related genes important for the survival of perennial plants in a seasonal climate. A full-length dehydrin gene (Dhn3) was characterised at the nucleotide level and the protein structure was modelled. Additionally, the allelic variation was analysed in five natural populations of Quercus petraea (Matt.) Liebl. sampled along an altitudinal gradient in the French Pyrenees. The analysed sequences contain typical domains of the K(n) class of dehydrins in the coding region. Also, the 5'untranslated region (promoter) of the gene was amplified, which shows typical motifs essential for drought- and cold-responsive gene expression. Single nucleotide substitutions and indels (insertions/deletions) within the coding region determine large biochemical differences at the protein level. However, only low levels of genetic differentiation between populations from different altitudes were detectable.
首次在常绿栎[Quercus petraea（Matt.）Liebl.]中，分离和鉴定了全长脱水素基因及其启动子区域，并在自然种群中分析了其等位基因变异。脱水素（Dhn）是与季节性气候中多年生植物生存相关的应激相关基因。对全长脱水素基因（Dhn3）进行了核苷酸水平的特征描述，并对其蛋白质结构进行了建模。此外，还对在法国比利牛斯山脉海拔梯度上采集的五个天然栎属[Quercus petraea（Matt.）Liebl.]自然种群进行了等位基因变异分析。分析的序列在编码区包含 K（n）类脱水素的典型结构域。此外，还扩增了基因的 5'非翻译区（启动子），该序列具有干旱和冷响应基因表达所必需的典型基序。编码区的单核苷酸取代和插入/缺失（indels）决定了蛋白质水平上的大生化差异。然而，在不同海拔的种群之间仅检测到低水平的遗传分化。
