Department of Chemistry, Indian Institute of Technology Madras, Chennai 600 036, India.
Langmuir. 2011 Nov 15;27(22):13461-7. doi: 10.1021/la203028s. Epub 2011 Oct 19.
The interaction of submicellar concentrations of various physiologically important unconjugated [sodium deoxycholate (NaDC), sodium cholate (NaC)] and conjugated [sodium glycodeoxycholate (NaGDC), sodium glycocholate (NaGC), sodium taurodeoxycholate (NaTDC), sodium taurocholate (NaTC)] bile salts with dipalmitoylphosphatidylcholine (DPPC) and dimyristoylphosphatidylcholine (DMPC) small unilamellar vesicles in solid gel (SG) and liquid crystalline (LC) phases was investigated using the excited-state prototropism of 1-naphthol. Steady-state and time-resolved fluorescence of the two excited-state prototropic forms of 1-naphthol indicate that submicellar bile salt concentration induces hydration of the lipid bilayer membrane into the core region. This hydration effect is a general phenomenon of the bile salts studied. The bilayer hydration efficiency of the bile salt follows the order NaDC > NaC > NaGDC > NaTDC > NaGC > NaTC for both DPPC and DMPC vesicles in their SG and LC phases.
采用 1-萘酚的激发态质子转移研究了亚胶束浓度的各种生理上重要的未结合[脱氧胆酸钠（NaDC），胆酸钠（NaC）]和结合[甘氨脱氧胆酸钠（NaGDC），甘胆酸钠（NaGC），牛磺脱氧胆酸钠（NaTDC），牛磺胆酸钠（NaTC）]胆汁盐与二棕榈酰磷脂酰胆碱（DPPC）和二肉豆蔻酰磷脂酰胆碱（DMPC）小单层囊泡在固体凝胶（SG）和液晶（LC）相中的相互作用。1-萘酚的两个激发态质子转移形式的稳态和时间分辨荧光表明，亚胶束胆盐浓度将脂质双层膜的水合作用诱导到核心区域。这种水合作用是研究的胆汁盐的普遍现象。对于 DPPC 和 DMPC 囊泡在其 SG 和 LC 相中的两种囊泡，胆汁盐的双层水合效率遵循 NaDC>NaC>NaGDC>NaTDC>NaGC>NaTC 的顺序。
