利用云杉 SO2-乙醇-水废醪液进行连续丙酮-丁醇-乙醇发酵。
Continuous acetone-butanol-ethanol fermentation using SO2-ethanol-water spent liquor from spruce.
机构信息
Aalto University School of Chemical Technology, Department of Biotechnology and Chemical Technology, POB 16100, 00076 Aalto, Finland.
出版信息
Bioresour Technol. 2011 Dec;102(23):10996-1002. doi: 10.1016/j.biortech.2011.09.034. Epub 2011 Sep 16.
SO2-ethanol-water (SEW) spent liquor from spruce chips was successfully used for batch and continuous production of acetone, butanol and ethanol (ABE). Initially, batch experiments were performed using spent liquor to check the suitability for production of ABE. Maximum concentration of total ABE was found to be 8.79 g/l using 4-fold diluted SEW liquor supplemented with 35 g/l of glucose. The effect of dilution rate on solvent production, productivity and yield was studied in column reactor consisting of immobilized Clostridium acetobutylicum DSM 792 on wood pulp. Total solvent concentration of 12 g/l was obtained at a dilution rate of 0.21 h(-1). The maximum solvent productivity (4.86 g/l h) with yield of 0.27 g/g was obtained at dilution rate of 0.64 h(-1). Further, to increase the solvent yield, the unutilized sugars were subjected to batch fermentation.
云杉木屑的 SO2-乙醇-水（SEW）废液成功地用于丙酮丁醇乙醇（ABE）的分批和连续生产。最初，使用废液进行分批实验以检查生产 ABE 的适宜性。使用 4 倍稀释的 SEW 废液补充 35 g/l 葡萄糖，发现总 ABE 的最大浓度为 8.79 g/l。在装有固定化丙酮丁醇梭菌 DSM 792 的木浆的柱状反应器中研究了稀释率对溶剂生产、生产力和产率的影响。在稀释率为 0.21 h（-1）时，获得了 12 g/l 的总溶剂浓度。在稀释率为 0.64 h（-1）时，获得了最大溶剂生产力（4.86 g/l h）和产率为 0.27 g/g。此外，为了提高溶剂产率，未利用的糖进行分批发酵。
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